Chloroplast ATP synthase restricts photosynthesis under fluctuating light in tomato but not in maize

Photosynthesis in fluctuating light requires coordinated adjustments of diffusion conductance and biochemical capacity, but the role of chloroplast ATP synthase activity (gH+) in dynamic photosynthesis is not well understood. In this study, we measured gas exchange, chlorophyll fluorescence and electrochromic shift signals in fluctuating light for leaves of tomato (Solanum lycopersicum) and maize (Zea mays). During the transition from sun to shade, simultaneous increases in gH+, effective quantum yield of PSII, and net CO2 assimilation rate (AN) occurred in tomato but uncoupled in maize, indicating that gH + limited AN during the sun-to-shade transition in tomato but not in maize. During the shade-to-sun transition, gH + increased simultaneously with stomatal conductance, mesophyll conductance and Rubisco carboxylation capacity in tomato, suggesting that gH+ is an overlooked factor affecting light induction of AN in tomato. By comparison, gH + maintained at high levels in maize and its AN was mainly restricted by stomatal conductance. Our results reveal that the kinetics of gH+ in fluctuating light differs between species, and chloroplast ATP synthase may be a potential target for improving dynamic photosynthesis in crops such as tomato. •The kinetics of gH + under fluctuating light differ between tomato and maize.•gH + limits AN during the sun-to-shade transition in tomato but not in maize.•gH + limits AN during photosynthetic induction in tomato but not in maize.•gH + coordinates with gs, gm and Vcamx to regulate AN under fluctuating light.•Chloroplast ATP synthase is a potential target for improving dynamic photosynthesis.