CHCHD10P80L knock-in zebrafish display a mild ALS-like phenotype
Mutations in the nuclear-encoded mitochondrial gene CHCHD10 have been observed in patients with a spectrum of diseases that include amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). To investigate the pathogenic nature of disease-associated variants of CHCHD10 we generated a zeb...
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Veröffentlicht in: | Experimental neurology 2024-12, Vol.382, p.114945, Article 114945 |
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Zusammenfassung: | Mutations in the nuclear-encoded mitochondrial gene CHCHD10 have been observed in patients with a spectrum of diseases that include amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). To investigate the pathogenic nature of disease-associated variants of CHCHD10 we generated a zebrafish knock-in (KI) model expressing the orthologous ALS-associated CHCHD10P80L variant (zebrafish: Chchd10P83L). Larval chchd10P83L/P83L fish displayed reduced Chchd10 protein expression levels, motor impairment, reduced survival and abnormal neuromuscular junctions (NMJ). These deficits were not accompanied by changes in transcripts involved in the integrated stress response (ISR), phenocopying previous findings in our knockout (chchd10−/−). Adult, 11-month old chchd10P83L/P83L zebrafish, displayed smaller slow- and fast-twitch muscle cell cross-sectional areas compared to wild type zebrafish muscle cells. Motoneurons in the spinal cord of chchd10P83L/P83L zebrafish displayed similar cross-sectional areas to that of wild type motor neurons and significantly fewer motor neurons were observed when compared to chchd2−/− adult spinal cords. Bulk RNA sequencing using whole spinal cords of 7-month old fish revealed transcriptional changes associated with neuroinflammation, apoptosis, amino acid metabolism and mt-DNA inflammatory response in our chchd10P83L/P83L model. The findings presented here, suggest that the CHCHD10P80L variant confers an ALS-like phenotype when expressed in zebrafish.
•Larval knock-in CHCHD10P80L zebrafish display motor impairment.•Larval CHCHD10P80L zebrafish have abnormal NMJs and reduced levels of Chchd10.•Adult CHCHD10P80L zebrafish display smaller fast- and slow-twitch muscle cells.•Adult CHCHD10P80L zebrafish display a trend of fewer spinal cord motoneurons.•Transcriptional changes in apoptosis and mtDNA inflammatory response |
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ISSN: | 0014-4886 1090-2430 1090-2430 |
DOI: | 10.1016/j.expneurol.2024.114945 |