The chromatin accessibility landscape of mouse oocytes during configuration transition

The transition of chromatin configuration in mammalian oocytes from a non‐surrounded nucleolus (NSN) to a surrounded nucleolus (SN) is critical for acquiring the developmental competence. However, the genomic and epigenomic features underlying this process remain poorly understood. In the present st...

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Veröffentlicht in:Cell proliferation 2025-01, Vol.58 (1), p.e13733-n/a
Hauptverfasser: Zhu, Shuai, Li, Jiashuo, Wang, Xiuwan, Jin, Yifei, Wang, Hengjie, An, Huiqing, Sun, Hongzheng, Han, Longsen, Shen, Bin, Wang, Qiang
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Sprache:eng
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Zusammenfassung:The transition of chromatin configuration in mammalian oocytes from a non‐surrounded nucleolus (NSN) to a surrounded nucleolus (SN) is critical for acquiring the developmental competence. However, the genomic and epigenomic features underlying this process remain poorly understood. In the present study, we first establish the chromatin accessibility landscape of mouse oocytes from NSN to SN stage. Through the integrative analysis of multi‐omics, we find that the establishment of DNA methylation in oocytes is independent of the dynamics of chromatin accessibility. In contrast, histone H3K4me3 status is closely associated with the dynamics of accessible regions during configuration transition. Furthermore, by focusing on the actively transcribed genes in NSN and SN oocytes, we discover that chromatin accessibility coupled with histone methylation (H3K4me3 and H3K27me3) participates in the transcriptional control during phase transition. In sum, our data provide a comprehensive resource for probing configuration transition in oocytes, and offer insights into the mechanisms determining chromatin dynamics and oocyte quality. This study elucidated the chromatin accessibility landscape across the non‐surrounded nucleolus (NSN) to surrounded nucleolus (SN) transition in oocyte configuration. It highlights the autonomy of DNA methylation from chromatin accessibility, contrasting with the dependency of H3K4me3. The interplay between chromatin accessibility and histone modification in transcription regulation during oocyte development is also explored.
ISSN:0960-7722
1365-2184
1365-2184
DOI:10.1111/cpr.13733