Encapsulation of Candida antarctica lipase B in metal–organic framework under ultrasound and using it to one-pot synthesis of 1,3,4,5-tetrasubstituted pyrazoles
Encapsulating the enzyme in metal–organic frameworks (MOFs) is a convenient method to prepare MOF-enzyme biocomposite. In this study, Candida antarctica lipase B (CAL-B) was chosen to immobilize in Cu-BTC MOF under ultrasound irradiation. CAL-B was immobilized in Cu-BTC under ultrasound at 21 kHz an...
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Veröffentlicht in: | Bioprocess and biosystems engineering 2024-12, Vol.47 (12), p.2027-2041 |
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Sprache: | eng |
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Zusammenfassung: | Encapsulating the enzyme in metal–organic frameworks (MOFs) is a convenient method to prepare MOF-enzyme biocomposite. In this study,
Candida antarctica
lipase B (CAL-B) was chosen to immobilize in Cu-BTC MOF under ultrasound irradiation. CAL-B was immobilized in Cu-BTC under ultrasound at 21 kHz and 11.4 W/cm
2
and incubation. 98% of CAL-B was immobilized in Cu-BTC with 99 U/mg activity (threefold more active than the free CAL-B). The prepared biocomposite was characterized using FT-IR, XRD, TGA, SEM, EDX, and BET. The thermal and solvent stability of CAL-B@Cu-BTC was investigated. It was found that at a temperature of 55 ℃, CAL-B@Cu-BTC maintains its activity even after 2 h of incubation. Furthermore, in the presence of 20% and 50% concentrations of MeCN, THF, and DMF, CAL-B@Cu-BTC was found to have an activity of over 80%. A prepared biocatalyst was used to synthesize 1,3,4,5-tetrasubstituted pyrazole derivatives (50–75%) in a one-pot vessel, by adding phenyl hydrazine hydrochlorides, benzaldehydes, and dimethyl acetylenedicarboxylate.
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ISSN: | 1615-7591 1615-7605 1615-7605 |
DOI: | 10.1007/s00449-024-03083-4 |