Enhancing Sensitivity in Nucleic Acid Detection via Collaborative Multiple Catalytic Cores in DNAzyme Nanomachines

We introduce a multicore DNA nanomachine (MDNM), utilizing four binary DNAzymes for nucleic acid detection without the need for a preamplification step. This innovation remarkably yields a reduction in limit of detection (LOD), over 5‐fold, as compared to single‐core systems. This reduces the required test time thus highlighting the potential of MDNM in advancing nucleic acid detection. This communication introduces a significant innovation in nucleic acid detection by utilizing four binary DNAzymes, which notably reduces the limit of detection (LOD) over 5‐fold compared to single‐core systems. This advancement not only lowers the required test time but also showcases the potential of the multicore DNA nanomachine (MDNM) to revolutionize the field of nucleic acid detection.