Physical radiation induced the yield of triterpenoids in hypha of Inonotus obliquus to increase

HSD-IO01, a new pure strain of I. obliquus, was isolated and purified from the sclerotium of I. obliquus of Daxing'an Mountains. Physical radiation-assisted liquid fermentation technology was explored to increase the triterpenoids yield of HSD-IO01. In the 100 mL optimized liquid fermentation system, the hypha dry weight of HSD-IO01 was 1.7734 g, and the triterpenoids yield was 43.43 mg. Yields of triterpenoids increased after induction with ultrasound, microwave, or UV light, respectively. Among them, ultrasonic treatment had the most remarkable induction effect. The yield of triterpenoids would be increased to 68.35 mg (57.38 %) when the HSD-IO01 was treated by 100 W ultrasonic for 45 min. Establishing ultrasonic-assisted liquid fermentation technology could further promote the detailed development and comprehensive utilization of I. obliquus resources. [Display omitted] •Isolation and identification of pure strains of Inonotus obliquus from its fruiting bodies.(HSD-IO01:PQ062132)•Determination of optimal culture conditions for liquid fermentation culture.•Ultrasonic, microwave and UV-induced high yield of triterpenoids in Inonotus obliquus.•Ultrasonic-assisted technology is stronger than microwave induction and ultraviolet induction.•Excessive physical strength inhibits the production of triterpenoids by Inonotus obliquus.