Preparation of ceftiofur-encapsulated hen-egg low-density lipoproteins and their antibacterial effects on intracellular Staphylococcus aureus
Hen egg low-density lipoprotein (heLDL), as alternative of serum-derived LDL, was used as drug delivery system of ceftiofur (CEF). The CEF-loaded hen egg low-density lipoprotein (CEF-heLDL) with complete apolipoprotein structure and high drug loading rate was synthesized, possesses suitable particle...
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| Veröffentlicht in: | International journal of biological macromolecules 2024-10, Vol.278 (Pt 4), p.134840, Article 134840 |
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| container_title | International journal of biological macromolecules |
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| creator | Zhao, Yi Mao, Wei Liu, Bo Wang, Yong-fei Zhang, Shuang-yi Guo, Li-li Qian, Ying-hong Gong, Zhi-guo Zhao, Jia-min Yang, Xiao-lin Qu, Gang-gang Hasi, Su-rong Bai, Yu-ting Cao, Jin-shan |
| description | Hen egg low-density lipoprotein (heLDL), as alternative of serum-derived LDL, was used as drug delivery system of ceftiofur (CEF). The CEF-loaded hen egg low-density lipoprotein (CEF-heLDL) with complete apolipoprotein structure and high drug loading rate was synthesized, possesses suitable particle size. CEF-heLDL undergoes cellular uptake and colocalizes with lysosomes in vitro. An intracellular infection model of the bovine endometrial epithelial cells and a coeliac-induced inflammation model of mice by Staphylococcus aureus (S. aureus) were established, and significantly lower intracellular S. aureus levels of CEF-heLDL group than CEF-free group (P |
| doi_str_mv | 10.1016/j.ijbiomac.2024.134840 |
| format | Article |
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The CEF-loaded hen egg low-density lipoprotein (CEF-heLDL) with complete apolipoprotein structure and high drug loading rate was synthesized, possesses suitable particle size. CEF-heLDL undergoes cellular uptake and colocalizes with lysosomes in vitro. An intracellular infection model of the bovine endometrial epithelial cells and a coeliac-induced inflammation model of mice by Staphylococcus aureus (S. aureus) were established, and significantly lower intracellular S. aureus levels of CEF-heLDL group than CEF-free group (P < 0.001) was observed. The antibacterial efficacy was sustained for 24 h. Up to 400 mg/kg of CEF-heLDL, 20 times the clinical practice, were intraperitoneally administrated, and no significant toxicity signs on mice were observed. HeLDLs is an effective, safe, and cheap drug carrier, and could also be used for transmembrane delivering other antibiotics.</description><identifier>ISSN: 0141-8130</identifier><identifier>ISSN: 1879-0003</identifier><identifier>EISSN: 1879-0003</identifier><identifier>DOI: 10.1016/j.ijbiomac.2024.134840</identifier><identifier>PMID: 39217040</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Anti-Bacterial Agents - chemistry ; Anti-Bacterial Agents - pharmacology ; Cattle ; Ceftiofur ; Cephalosporins - chemistry ; Cephalosporins - pharmacokinetics ; Cephalosporins - pharmacology ; Chickens ; Drug Carriers - chemistry ; Eggs ; Female ; Hen-egg low-density lipoprotein ; Intracellular drug delivery system ; Lipoproteins, LDL - metabolism ; Mice ; Staphylococcal Infections - drug therapy ; Staphylococcus aureus ; Staphylococcus aureus - drug effects</subject><ispartof>International journal of biological macromolecules, 2024-10, Vol.278 (Pt 4), p.134840, Article 134840</ispartof><rights>2024 Elsevier B.V.</rights><rights>Copyright © 2024 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c245t-357c366623a7ffe7635558bb0762f59a3bd0bf4417f46c454074676540769f513</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0141813024056459$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39217040$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhao, Yi</creatorcontrib><creatorcontrib>Mao, Wei</creatorcontrib><creatorcontrib>Liu, Bo</creatorcontrib><creatorcontrib>Wang, Yong-fei</creatorcontrib><creatorcontrib>Zhang, Shuang-yi</creatorcontrib><creatorcontrib>Guo, Li-li</creatorcontrib><creatorcontrib>Qian, Ying-hong</creatorcontrib><creatorcontrib>Gong, Zhi-guo</creatorcontrib><creatorcontrib>Zhao, Jia-min</creatorcontrib><creatorcontrib>Yang, Xiao-lin</creatorcontrib><creatorcontrib>Qu, Gang-gang</creatorcontrib><creatorcontrib>Hasi, Su-rong</creatorcontrib><creatorcontrib>Bai, Yu-ting</creatorcontrib><creatorcontrib>Cao, Jin-shan</creatorcontrib><title>Preparation of ceftiofur-encapsulated hen-egg low-density lipoproteins and their antibacterial effects on intracellular Staphylococcus aureus</title><title>International journal of biological macromolecules</title><addtitle>Int J Biol Macromol</addtitle><description>Hen egg low-density lipoprotein (heLDL), as alternative of serum-derived LDL, was used as drug delivery system of ceftiofur (CEF). The CEF-loaded hen egg low-density lipoprotein (CEF-heLDL) with complete apolipoprotein structure and high drug loading rate was synthesized, possesses suitable particle size. CEF-heLDL undergoes cellular uptake and colocalizes with lysosomes in vitro. An intracellular infection model of the bovine endometrial epithelial cells and a coeliac-induced inflammation model of mice by Staphylococcus aureus (S. aureus) were established, and significantly lower intracellular S. aureus levels of CEF-heLDL group than CEF-free group (P < 0.001) was observed. The antibacterial efficacy was sustained for 24 h. Up to 400 mg/kg of CEF-heLDL, 20 times the clinical practice, were intraperitoneally administrated, and no significant toxicity signs on mice were observed. HeLDLs is an effective, safe, and cheap drug carrier, and could also be used for transmembrane delivering other antibiotics.</description><subject>Animals</subject><subject>Anti-Bacterial Agents - chemistry</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Cattle</subject><subject>Ceftiofur</subject><subject>Cephalosporins - chemistry</subject><subject>Cephalosporins - pharmacokinetics</subject><subject>Cephalosporins - pharmacology</subject><subject>Chickens</subject><subject>Drug Carriers - chemistry</subject><subject>Eggs</subject><subject>Female</subject><subject>Hen-egg low-density lipoprotein</subject><subject>Intracellular drug delivery system</subject><subject>Lipoproteins, LDL - metabolism</subject><subject>Mice</subject><subject>Staphylococcal Infections - drug therapy</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus - drug effects</subject><issn>0141-8130</issn><issn>1879-0003</issn><issn>1879-0003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUctuFDEQtBARWRJ-IfKRyyzt8WvmBop4SZFAIjlbHk8769XseLA9oP0I_hmvNuHKqVutquruKkJuGGwZMPVuvw37IcSDddsWWrFlXHQCXpAN63TfAAB_STbABGs6xuGSvM55X6dKsu4VueR9yzQI2JA_3xMuNtkS4kyjpw59bf2aGpydXfI62YIj3eHc4OMjneLvZsQ5h3KkU1jikmLBMGdq55GWHYZUuxIG6wqmYCeK3qMrmVb1MJdkHU5T1Uz0R7HL7jhFF51bK39NuOZrcuHtlPHNU70iD58-3t9-ae6-ff56--Guca2QpeFSO66UarnVVV8rLqXshgG0ar3sLR9GGLwQTHuhnJACtFBanarqvWT8irw969b7f66YizmEfDrNzhjXbDj0fQcdMF6h6gx1Keac0JslhYNNR8PAnKIwe_MchTlFYc5RVOLN0451OOD4j_bsfQW8PwOwfvorYDLZheo6jiFVz8wYw_92_AXuHKBQ</recordid><startdate>202410</startdate><enddate>202410</enddate><creator>Zhao, Yi</creator><creator>Mao, Wei</creator><creator>Liu, Bo</creator><creator>Wang, Yong-fei</creator><creator>Zhang, Shuang-yi</creator><creator>Guo, Li-li</creator><creator>Qian, Ying-hong</creator><creator>Gong, Zhi-guo</creator><creator>Zhao, Jia-min</creator><creator>Yang, Xiao-lin</creator><creator>Qu, Gang-gang</creator><creator>Hasi, Su-rong</creator><creator>Bai, Yu-ting</creator><creator>Cao, Jin-shan</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202410</creationdate><title>Preparation of ceftiofur-encapsulated hen-egg low-density lipoproteins and their antibacterial effects on intracellular Staphylococcus aureus</title><author>Zhao, Yi ; Mao, Wei ; Liu, Bo ; Wang, Yong-fei ; Zhang, Shuang-yi ; Guo, Li-li ; Qian, Ying-hong ; Gong, Zhi-guo ; Zhao, Jia-min ; Yang, Xiao-lin ; Qu, Gang-gang ; Hasi, Su-rong ; Bai, Yu-ting ; Cao, Jin-shan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c245t-357c366623a7ffe7635558bb0762f59a3bd0bf4417f46c454074676540769f513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Animals</topic><topic>Anti-Bacterial Agents - chemistry</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Cattle</topic><topic>Ceftiofur</topic><topic>Cephalosporins - chemistry</topic><topic>Cephalosporins - pharmacokinetics</topic><topic>Cephalosporins - pharmacology</topic><topic>Chickens</topic><topic>Drug Carriers - chemistry</topic><topic>Eggs</topic><topic>Female</topic><topic>Hen-egg low-density lipoprotein</topic><topic>Intracellular drug delivery system</topic><topic>Lipoproteins, LDL - metabolism</topic><topic>Mice</topic><topic>Staphylococcal Infections - drug therapy</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus aureus - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhao, Yi</creatorcontrib><creatorcontrib>Mao, Wei</creatorcontrib><creatorcontrib>Liu, Bo</creatorcontrib><creatorcontrib>Wang, Yong-fei</creatorcontrib><creatorcontrib>Zhang, Shuang-yi</creatorcontrib><creatorcontrib>Guo, Li-li</creatorcontrib><creatorcontrib>Qian, Ying-hong</creatorcontrib><creatorcontrib>Gong, Zhi-guo</creatorcontrib><creatorcontrib>Zhao, Jia-min</creatorcontrib><creatorcontrib>Yang, Xiao-lin</creatorcontrib><creatorcontrib>Qu, Gang-gang</creatorcontrib><creatorcontrib>Hasi, Su-rong</creatorcontrib><creatorcontrib>Bai, Yu-ting</creatorcontrib><creatorcontrib>Cao, Jin-shan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of biological macromolecules</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhao, Yi</au><au>Mao, Wei</au><au>Liu, Bo</au><au>Wang, Yong-fei</au><au>Zhang, Shuang-yi</au><au>Guo, Li-li</au><au>Qian, Ying-hong</au><au>Gong, Zhi-guo</au><au>Zhao, Jia-min</au><au>Yang, Xiao-lin</au><au>Qu, Gang-gang</au><au>Hasi, Su-rong</au><au>Bai, Yu-ting</au><au>Cao, Jin-shan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparation of ceftiofur-encapsulated hen-egg low-density lipoproteins and their antibacterial effects on intracellular Staphylococcus aureus</atitle><jtitle>International journal of biological macromolecules</jtitle><addtitle>Int J Biol Macromol</addtitle><date>2024-10</date><risdate>2024</risdate><volume>278</volume><issue>Pt 4</issue><spage>134840</spage><pages>134840-</pages><artnum>134840</artnum><issn>0141-8130</issn><issn>1879-0003</issn><eissn>1879-0003</eissn><abstract>Hen egg low-density lipoprotein (heLDL), as alternative of serum-derived LDL, was used as drug delivery system of ceftiofur (CEF). The CEF-loaded hen egg low-density lipoprotein (CEF-heLDL) with complete apolipoprotein structure and high drug loading rate was synthesized, possesses suitable particle size. CEF-heLDL undergoes cellular uptake and colocalizes with lysosomes in vitro. An intracellular infection model of the bovine endometrial epithelial cells and a coeliac-induced inflammation model of mice by Staphylococcus aureus (S. aureus) were established, and significantly lower intracellular S. aureus levels of CEF-heLDL group than CEF-free group (P < 0.001) was observed. The antibacterial efficacy was sustained for 24 h. Up to 400 mg/kg of CEF-heLDL, 20 times the clinical practice, were intraperitoneally administrated, and no significant toxicity signs on mice were observed. HeLDLs is an effective, safe, and cheap drug carrier, and could also be used for transmembrane delivering other antibiotics.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>39217040</pmid><doi>10.1016/j.ijbiomac.2024.134840</doi></addata></record> |
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| subjects | Animals Anti-Bacterial Agents - chemistry Anti-Bacterial Agents - pharmacology Cattle Ceftiofur Cephalosporins - chemistry Cephalosporins - pharmacokinetics Cephalosporins - pharmacology Chickens Drug Carriers - chemistry Eggs Female Hen-egg low-density lipoprotein Intracellular drug delivery system Lipoproteins, LDL - metabolism Mice Staphylococcal Infections - drug therapy Staphylococcus aureus Staphylococcus aureus - drug effects |
| title | Preparation of ceftiofur-encapsulated hen-egg low-density lipoproteins and their antibacterial effects on intracellular Staphylococcus aureus |
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