Efficient cadmium-resistant plant growth-promoting bacteria loaded on pig bone biochar has higher efficiency in reducing cadmium phytoavailability and improving maize performance than on rice husk biochar

Green agriculture faced challenges due to the shortage of efficient cadmium (Cd)-resistant plant growth-promoting bacteria (CdR-PGPB) and their low survival rate and activity during application. In this study, a diverse range of efficient CdR-PGPB were isolated from the rhizosphere soil of Desmodium elegans, especially those with high phosphate-solubilizing capabilities (272.87–450.45 mg L−1). Two highly efficient CdR-PGPB namely, XH1 and XH3 were loaded on to rice husk biochar (RHB) and pig bone biochar (PBB), labelled as RHBM and PBBM respectively. This study aimed to explore their effectiveness and mechanisms in promoting maize growth in a Cd-contaminated planting system. Results showed that PBBM performed best among all treatments. It significantly decreased soil phytoavailable Cd by 53.19 % and Cd content in maize shoot by 85.89 %. It also increased soil available phosphorus by 145.72 %, soil alkaline phosphatase activity by 76.34 %, maize shoot/root biomass by 47.06 %/67.98 %, Chlorophyll (a/b) content by 66.80 %/134.13 % and peroxidase activity by 171.96 %. These results were achieved through the synergistic action of efficient CdR-PGPB and PBB. Therefore, PBBM proved to be a promising and innovative application technique for sustainable agricultural development in Cd-contaminated farmland ecosystems. [Display omitted] •Rich CdR-PGPB with high plant growth-promoting capacity were isolated from the rhizosphere soil of Desmodium elegans.•Compared with RHB, PBB provided CdR-PGPB with more nutrients and habitats.•PBBM demonstrated superior performance in reducing cadmium phytoavailability and improving soil fertility and maize growth.