Enhanced Osteogenesis in 2D and 3D Culture Systems Using RGD Peptide and α‐TCP Phase Transition within Alginate‐Based Hydrogel

Cell‐laden hydrogels have been extensively investigated in various tissue engineering fields by their potential capacity to deposit numerous types of cells in a specific area. They are largely used in soft‐tissue engineering applications because of their low mechanical strength. In addition, sodium alginate is well‐known for its encapsulation, loading capacity and for being easily controllable; however, it lacks cell‐binding ligands and hence the ability to adhere cells. In this study, it is aimed to enhance osteogenesis in cells encapsulated in alginate and improve its mechanical properties by introducing a synthetic peptide and calcium phosphate phase transition. To increase cell–hydrogel interactions and increasing cell viability, an RGD peptide is added to a photocrosslinkable methacrylate‐modified alginate, and alpha‐tricalcium phosphate (α‐TCP) is added to the hydrogel to increase its mechanical strength via phase transition. Cell proliferation, growth, and differentiation are assessed in both 2D and 3D cell cultures. The addition of α‐TCP significantly improved the mechanical properties of the hydrogel. Moreover, the RGD peptide and α‐TCP showed a synergistic effect with significantly improved cell adhesion and osteogenesis in both 2D and 3D cell cultures. Therefore, the functional hydrogel developed in this study can potentially be used for bone tissue regeneration. Cell‐laden hydrogels, used in soft‐tissue engineering for their cell deposition capabilities, often lack cell‐binding ligands. This study enhanced alginate hydrogels' mechanical properties and osteogenesis by adding an RGD peptide and alpha‐tricalcium phosphate, improving cell adhesion and viability in 2D and 3D cultures.