Toothpick DNA extraction combined with handheld LAMP microfluidic platform for simple and rapid meat authentication

Adulteration of meat is a global issue, necessitating rapid, inexpensive, and simple on-site testing methods. Therefore, the present study aimed to develop a one-minute toothpick-based DNA extraction method, a handheld microfluidic chip, and a smartphone-controlled portable analyzer for detecting multiple meat adulterations. A toothpick was inserted into the meat to promote DNA release and adsorption. Furthermore, a handheld microfluidic chip was designed for DNA elution on toothpicks and fluid distribution. Finally, a smartphone-actuated portable analyzer was developed to function as a heater, signal detector, and result reader. The portable device comprises a microcontroller, a fluorescence detection module, a step scanning unit, and a heating module. The proposed device is portable, and the app is user-friendly. This simple design, easy operation, and fast-response system could rapidly detect as little as 1% of simulated adulterated samples (following UK standards) within 40 min at a cost of less than USD 1 per test. •Toothpick DNA extraction was developed for meat adulteration detection within 1 min.•Low-cost reagents were embedded on-chip to simplify the operation process.•A detector function as a heater, signal detector, and result reader.•This platform enabled real-time data display and store.•Simulated and commercially adulterated samples were detected by the system.