(+)‐Catechin attenuates CCI‐induced neuropathic pain in male rats by promoting the Nrf2 antioxidant pathway to inhibit ROS/TLR4/NF‐κB‐mediated activation of the NLRP3 inflammasome

The objective of this study was to investigate the potential mechanisms by which (+)‐catechin alleviates neuropathic pain. Thirty‐two male Sprague–Dawley rats were divided into four groups: the sham group, the chronic constriction injury (CCI)group, the CCI+ ibuprofen group, and the CCI+ (+)‐catechi...

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Veröffentlicht in:Journal of neuroscience research 2024-08, Vol.102 (8), p.e25372-n/a
Hauptverfasser: Jing, Bei, Chen, Zhen‐ni, Si, Wai‐mei, Zhao, Jia‐ji, Zhao, Guo‐ping, Zhang, Di
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Sprache:eng
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Zusammenfassung:The objective of this study was to investigate the potential mechanisms by which (+)‐catechin alleviates neuropathic pain. Thirty‐two male Sprague–Dawley rats were divided into four groups: the sham group, the chronic constriction injury (CCI)group, the CCI+ ibuprofen group, and the CCI+ (+)‐catechin group. CCI surgery induces thermal hyperalgesia in rats and (+)‐catechin ameliorated CCI‐induced thermal hyperalgesia and repaired damaged sciatic nerve in rats. CCI decreased SOD levels in male rat spinal cord dorsal horn and promoted MDA production, induced oxidative stress by increasing NOX4 levels and decreasing antioxidant enzyme HO‐1 levels, and also increased protein levels of TLR4, p‐NF‐κB, NLRP3 inflammasome components, and IL‐1β. In contrast, (+)‐catechin reversed the above results. In i vitro experiments, (+)‐catechin reduced the generation of reactive oxygen species (ROS) in GMI‐R1 cells after LPS stimulation and attenuated the co‐expression of IBA‐1 and NLRP3. It also showed significant inhibition of the NF‐κB and NLRP3 inflammatory pathways and activation of the Nrf2‐mediated antioxidant system. Overall, these findings suggest that (+)‐catechin inhibits the activation of the NLRP3 inflammasome through the triggering of the Nrf2‐induced antioxidant system, the inhibition of the TLR4/NF‐κB pathway, and the production of ROS to alleviate CCI‐induced neuropathic pain in male rats. +)‐Catechin exerts neuroprotective effects by triggering the Nrf2‐induced antioxidant system and attenuates NLRP3 inflammatory vesicle activation through inhibition of ROS production and the TLR4/NF‐κB pathway, thereby alleviating CCI‐induced neuropathic pain in male rats.
ISSN:0360-4012
1097-4547
1097-4547
DOI:10.1002/jnr.25372