Genome-Wide Identification and Expression Analysis of ADK Gene Family Members in Cotton under Abiotic Stress
Adenosine kinase (ADK) is a key enzyme widely distributed in plants, playing an important role in maintaining cellular energy homeostasis and regulating plant growth, development, and responses to environmental stresses. However, research on genes in cotton ( ), an economically significant crop, has...
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Veröffentlicht in: | International journal of molecular sciences 2024-07, Vol.25 (14), p.7821 |
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Sprache: | eng |
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Zusammenfassung: | Adenosine kinase (ADK) is a key enzyme widely distributed in plants, playing an important role in maintaining cellular energy homeostasis and regulating plant growth, development, and responses to environmental stresses. However, research on
genes in cotton (
), an economically significant crop, has been limited. This study identified 92
genes from four cotton species (
,
,
, and
) using HMMER and Local BLASTP methods and classified them into six groups. Chromosomal localization revealed a random distribution of
genes in
, with 13 genes located on the At subgenome and 14 genes on the Dt subgenome. Gene structure analysis showed consistency in exon-intron organization within subgroups, while conserved motif analysis identified subgroup-specific motifs, indicating functional diversity. Synteny and collinearity mapping analysis revealed that the primary expansion mechanisms of the
gene family in cotton are polyploidy and segmental duplication. Cis-regulatory elements in
promoters were classified into light response, hormone response, developmental regulation, and stress response. We also analyzed the expression patterns of
genes under a low temperature (4 °C) and drought conditions. Most
genes responded to cold stress with different expression patterns, indicating their roles in rapid response and long-term cold adaptation. Under drought stress, expression patterns varied, with some genes showing sustained high expression levels. The qRT-PCR validation of transcriptomic data confirmed the stress-induced expression patterns of selected
genes. Functional analysis through the VIGS silencing of
demonstrated its importance in cold and drought stress responses, with silencing resulting in poor growth under stress, highlighting its significance in stress tolerance. This study provides a basis for further understanding the evolutionary relationships and functions of the cotton
gene family. |
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ISSN: | 1422-0067 1661-6596 1422-0067 |
DOI: | 10.3390/ijms25147821 |