Identification and Expression Analysis of UPS Gene Family in Potato

Ureide permeases (UPSs) mediate the transport of ureides, including allantoin and allantoate, which act as nitrogen-transporting compounds in plants and have recently been found to play a role in cellular signaling. To date, UPSs have not been reported in potato, and their identification is important for further function studies and for understanding molecular mechanisms of plant adverse responses. Based on potato genomic data, we identified 10 genes in potato ( L.). Then, we conducted a comprehensive study of the identified genes using bioinformatics methods. Genome phylogenetic and genomic localization analyses revealed that can be classified into four categories, are highly homologous to UPS members, and are distributed on three chromosomes. The six genes were investigated by RT-qPCR, and the findings indicated that all of these genes are involved in the response to several stresses, including low nitrogen, cold, ABA, salt, H O and drought. This study establishes a strong theoretical framework for investigating the function of potato genes, as well as the molecular mechanisms underlying the responses of these genes to various environmental stresses.