Nanozymes for biomedical applications: Multi‐metallic systems may improve activity but at the cost of higher toxicity?

Nanozymes are nanomaterials with intrinsic enzyme‐like activity with selected advantages over native enzymes such as simple synthesis, controllable activity, high stability, and low cost. These materials have been explored as surrogates to natural enzymes in biosensing, therapeutics, environmental protection, and many other fields. Among different nanozymes classes, metal‐ and metal oxide‐based nanozymes are the most widely studied. In recent years, bi‐ and tri‐metallic nanomaterials have emerged often showing improved nanozyme activity, some of which even possess multifunctional enzyme‐like activity. Taking this concept even further, high‐entropy nanomaterials, that is, complex multicomponent alloys and ceramics like oxides, may potentially enhance activity even further. However, the addition of various elements to increase catalytic activity may come at the cost of increased toxicity. Since many nanozyme compositions are currently being explored for in vivo biomedical applications, such as cancer therapeutics, toxicity considerations in relation to nanozyme application in biomedicine are of vital importance for translation. This article is categorized under: Therapeutic Approaches and Drug Discovery > Emerging Technologies Toxicology and Regulatory Issues in Nanomedicine > Toxicology of Nanomaterials Diagnostic Tools > Diagnostic Nanodevices Nanozymes are inexpensive, stable and versatile alternatives to natural enzymes. They are under investigation for a variety of biomedical applications. Multi‐metallic systems including high entropy materials may significantly improve nanozyme activity, but do they come at the cost of higher toxicity?