Phenylobacterium montanum sp. nov., an oligotrophic, slightly acidophilic mesophile isolated from sandy soil

A bacterial strain, designated S6 , was isolated from the sandy soil on a rocky mountain in South China. Cells of S6 were Gram-stain-negative, aerobic, non-spore-forming, non-motile and non-prosthecae-producing. 16S rRNA gene sequence analysis revealed the highest similarities to 12 uncultured bacteria, followed by sp. B6.10-61 (97.14 %). The closest related validly published strains are ATCC 15253 (96.15 %), FWC 21 (96.08 %) and FWC 38 (96.08 %). Phylogenetic analysis based on 16S rRNA gene, genome and proteome sequences demonstrated that S6 formed a separated lineage in the genus . Strain S6 contained Q-10 (97.5 %) as the major ubiquinone and C ω7 and C as the major fatty acids. The polar lipid profile consisted of phosphatidylglycerol, an unknown phosphoglycolipid and three unknown glycolipids. The assembled genome comprises a chromosome with a length of 5.5 Mb and a plasmid of 96 014 bp. The G+C content was 67.6 mol%. The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished this strain from its closest phylogenetic neighbours. Thus it is proposed that strain S6 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is S6 (=NBRC 115419 =GCMCC 1.18594 ).