Escherichia coli grown in inexpensive conical flat-bottom polypropylene tubes produce a high level of pUC vector

The pUC-derived plasmid yield from E. coli using polypropylene tubes (PP) was compared among round and conical tubes. The yield from cells grown in a cheaper conical-PP with flat-bottom was 1.5-fold higher (p 

Bibliographische Detailangaben

Veröffentlicht in:	Journal of microbiological methods 2024-09, Vol.224, p.106990, Article 106990
Hauptverfasser:	Marquez, Mayra, Chen, Qian, Cachaco, Silvia, Yang, Jun, Sui, Hongyan, Imamichi, Tomozumi
Format:	Artikel
Sprache:	eng
Schlagworte:	Conical tubes Escherichia coli - genetics Escherichia coli - growth & development Genetic Vectors Isolation Laboratory budget Plasmid DNA Plasmids - genetics Polypropylene Polypropylenes - chemistry Polystyrene
Online-Zugang:	Volltext
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