Escherichia coli grown in inexpensive conical flat-bottom polypropylene tubes produce a high level of pUC vector

Escherichia coli grown in inexpensive conical flat-bottom polypropylene tubes produce a high level of pUC vector

The pUC-derived plasmid yield from E. coli using polypropylene tubes (PP) was compared among round and conical tubes. The yield from cells grown in a cheaper conical-PP with flat-bottom was 1.5-fold higher (p 

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Veröffentlicht in:Journal of microbiological methods 2024-09, Vol.224, p.106990, Article 106990
Hauptverfasser: Marquez, Mayra, Chen, Qian, Cachaco, Silvia, Yang, Jun, Sui, Hongyan, Imamichi, Tomozumi
Format: Artikel
Sprache:eng
Schlagworte:
Conical tubes
Escherichia coli - genetics
Escherichia coli - growth & development
Genetic Vectors
Isolation
Laboratory budget
Plasmid DNA
Plasmids - genetics
Polypropylene
Polypropylenes - chemistry
Polystyrene
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Zusammenfassung:The pUC-derived plasmid yield from E. coli using polypropylene tubes (PP) was compared among round and conical tubes. The yield from cells grown in a cheaper conical-PP with flat-bottom was 1.5-fold higher (p 
ISSN:0167-7012
1872-8359
1872-8359
DOI:10.1016/j.mimet.2024.106990