The dominant-substrate driven the enhanced performance in co-digestion of Pennisetum hybrid and livestock waste

Co-digestion has been considered a promising method to improve methane yield. The effect of the proportion of dominant substrate on the performance and microbial community of anaerobic digestion of Pennisetum hybrid (PH) and livestock waste (LW) was investigated. An obvious synergistic effect was obtained with an increase of 15.20%–17.45% in specific methane yield compared to the predicted value. Meanwhile, the dominant substrate influenced the relational model between methane yield enhancement rate and mixture ratio. For the LW-dominant systems, a parabolic model between enhancement rate and mixture ratio was observed with a highest value of 392.16 mL/g VS achieved at a PH:LW ratio of 2:8. While a linear pattern appeared for PH-dominant systems with the highest methane yield of 307.59 mL/g VS. Co-digestion selectively enriched the relative abundance of Clostridium_sensu_stricto_1, Terrisporobacter, Syntrophomonas, Methanosarcina and Methanobacterium, which boosted the performance of hydrolysis, acidogenesis, acetogenesis and methanogenesis processes. [Display omitted] •Co-digestion of Pennisetum hybrid and livestock waste boosts the AD performance.•Dominant substrate had a decisive effect on the change trend of methane enhancement.•An increase of 3.29%–213.87% in specific methane yield obtained for co-digestion.•Co-digestion selectively enriched the lignocellulose-degrading microorganisms.•Acetoclastic methanogenesis pathway dominated, followed by hydrogenotrophic-type.