Molecular evolution of Hokkaido virus, a genotype of Orthohantavirus puumalaense, among Myodes rodents

Viruses in the genus Orthohantavirus within the family Hantaviridae cause human hantavirus infections and represent a threat to public health. Hokkaido virus (HOKV), a genotype of Orthohantavirus puumalaense (Puumala virus; PUUV), was first identified in Tobetsu, Hokkaido, Japan. Although it is genetically related to the prototype of PUUV, the evolutionary pathway of HOKV is unclear. We conducted a field survey in a forest in Tobetsu in 2022 and captured 44 rodents. Complete coding genome sequences of HOKVs were obtained from five viral-RNA-positive rodents (four Myodes rufocanus bedfordiae and one Apodemus speciosus). Phylogenetic analysis revealed a close relationship between the phylogenies and geographical origins of M. rufocanus-related orthohantaviruses. Comparison of the phylogenetic trees of the S segments of orthohantaviruses and the cytochrome b genes of Myodes species suggested that Myodes-related orthohantaviruses evolved in Myodes rodent species as a result of genetic isolation and host switching. •A field survey was conducted in a forest in Tobetsu, Hokkaido, Japan in 2022 and 44 rodents were captured.•Complete coding genome sequences of Hokkaido virus (HOKV), a genotype of Orthohantavirus puumalaense were obtained from five viral-RNA-positive rodents.•Phylogenetic analysis revealed a close relationship between the phylogenies and geographical origins of M. rufocanus-related orthohantaviruses.•Orthohantaviruses have evolved in Myodes rodent species by genetic isolation of the host and host switching of the virus.