Sequencing a CC239-MRSA-III with a novel composite SCC mec element from Kuwait
Staphylococcus aureus CC239-MRSA-III is an ancient pandemic strain of hospital-associated, methicillin-resistant S. aureus that spread globally for decades and that still can be found in some parts of the world. In Kuwait, microarray-based surveillance identified from 2019 to 2022 a series of isolat...
Gespeichert in:
Veröffentlicht in: | European journal of clinical microbiology & infectious diseases 2024-09, Vol.43 (9), p.1761-1775 |
---|---|
Hauptverfasser: | , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | Staphylococcus aureus
CC239-MRSA-III is an ancient pandemic strain of hospital-associated, methicillin-resistant
S. aureus
that spread globally for decades and that still can be found in some parts of the world. In Kuwait, microarray-based surveillance identified from 2019 to 2022 a series of isolates of a hitherto unknown variant of this strain that carried a second set of recombinase genes,
ccrA/B-2
. To elucidate the structure of its SCC
mec
element, two isolates were subjected to nanopore sequencing. This revealed, in addition to
ccrA/B-2
, several SCC-associated genes including
speG
(spermidine N acetyltransferase) and a gene encoding a large “E-domain containing protein” (dubbed as
edcP-
SCC). This gene contained three regions consisting of multiple repeating units. In terms of sequence and structure it was similar but not identical to the biofilm-related
aap
gene from
S. epidermidis.
A review of published sequences identified
edcP-
SCC in eighteen genome sequences of
S. aureus, S. epidermidis
and
S. capitis
, and frequently it appears in a similar cluster of genes as in the strains sequenced herein. Isolates also carried a prophage with the adhesion factor
sasX/sesI
and aminoglycoside resistance genes. This is consistent with an affiliation to the “South-East Asian” Clade of CC239. The emergence of
edcP
-SCC and
sasX
-positive CC239 strain shows that, against a global trend towards community-associated MRSA, the ancient pandemic CC239 hospital strain still continues to evolve and to cause outbreaks. |
---|---|
ISSN: | 0934-9723 1435-4373 1435-4373 |
DOI: | 10.1007/s10096-024-04891-y |