Microglia are dispensable for experience-dependent refinement of mouse visual circuitry

To test the hypothesized crucial role of microglia in the developmental refinement of neural circuitry, we depleted microglia from mice of both sexes with PLX5622 and examined the experience-dependent maturation of visual circuitry and function. We assessed retinal function, receptive field tuning of visual cortex neurons, acuity and experience-dependent plasticity. None of these measurements detectibly differed in the absence of microglia, challenging the role of microglia in sculpting neural circuits. The authors test the model that microglia are crucial for the developmental refinement of neural circuitry by depleting them with PLX5622. Microglia prove dispensable for the experience-dependent maturation of visual circuitry during development.