Therapeutic Inhibition of LincRNA-p21 Protects Against Cardiac Hypertrophy

Cardiac hypertrophy is an adaptive response to pressure overload aimed at maintaining cardiac function. However, prolonged hypertrophy significantly increases the risk of maladaptive cardiac remodeling and heart failure. Recent studies have implicated long noncoding RNAs in cardiac hypertrophy and cardiomyopathy, but their significance and mechanism(s) of action are not well understood. We measured RNA and H3K27ac levels in the hearts of dilated cardiomyopathy patients. We assessed the functional role of in basal and surgical pressure-overload conditions using loss-of-function mice. Genome-wide transcriptome analysis revealed dysregulated genes and pathways. We labeled proteins in proximity to full-length using a novel BioID2-based system. We immunoprecipitated -interacting proteins and performed cell fractionation, ChIP-seq (chromatin immunoprecipitation followed by sequencing), and co-immunoprecipitation to investigate molecular interactions and underlying mechanisms. We used GapmeR antisense oligonucleotides to evaluate the therapeutic potential of inhibition in cardiac hypertrophy and associated heart failure. was induced in mice and humans with cardiomyopathy. Global and cardiac-specific knockout significantly suppressed pressure overload-induced ventricular wall thickening, stress marker elevation, and deterioration of cardiac function. Genome-wide transcriptome analysis and transcriptional network analysis revealed that acts to stimulate the NFAT/MEF2 pathway. Mechanistically, is bound to the scaffold protein KAP1. cardiac-specific knockout suppressed stress-induced nuclear accumulation of KAP1, and KAP1 knockdown attenuated cardiac hypertrophy and NFAT activation. KAP1 positively regulates pathological hypertrophy by physically interacting with NFATC4 to promote the overactive status of NFAT/MEF2 signaling. GapmeR antisense oligonucleotide depletion of similarly inhibited cardiac hypertrophy and adverse remodeling, highlighting the therapeutic potential of inhibiting . These findings advance our understanding of the functional significance of stress-induced long noncoding RNA in cardiac hypertrophy and demonstrate the potential of as a novel therapeutic target for cardiac hypertrophy and subsequent heart failure.