Application of LAMP assay for detection of carbapenem-resistant Acinetobacter calcoaceticus-Acinetobacter baumannii complex in ICU admitted sepsis patients: A rapid and cost-effective diagnostic tool

•Rapid diagnosis of sepsis is important to initiate early optimal treatment.•LAMP assay was performed to detect CR-SM-ACB complex from patients with sepsis.•Assay sensitivity, specificity, and accuracy were compared with RealAmp and PCR.•LAMP assay can be used to detect pathogens from direct positiv...

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Veröffentlicht in:	Diagnostic microbiology and infectious disease 2024-09, Vol.110 (1), p.116398, Article 116398
Hauptverfasser:	Sharma, Amit, Azam, Mudsser, Verma, P.K., Talwar, Vandana, Roy, Subhasree, Veeraraghavan, Balaji, Singh, Ruchi, Gaind, Rajni
Format:	Artikel
Sprache:	eng
Schlagworte:	blaOXA-23 mediated carbapenem resistance Internal transcribing spacer (ITS) 16S-23S rRNA Loop-mediated isothermal amplification (LAMP) assay Positive blood culture broth Sepsis Significant members of Acinetobacter calcoaceticus–Acinetobacter baumannii (SM-ACB) complex
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Zusammenfassung:	•Rapid diagnosis of sepsis is important to initiate early optimal treatment.•LAMP assay was performed to detect CR-SM-ACB complex from patients with sepsis.•Assay sensitivity, specificity, and accuracy were compared with RealAmp and PCR.•LAMP assay can be used to detect pathogens from direct positive culture broths.•The TAT of LAMP assay was
ISSN:	0732-8893 1879-0070 1879-0070
DOI:	10.1016/j.diagmicrobio.2024.116398