Ultrasensitive dynamic light scattering immunodetection of alpha-fetoprotein using heptamer-amplified nanoparticle crosslinking aggregation
A novel construction strategy is introduced for an ultrasensitive dynamic light scattering (DLS) immunosensor targeting alpha fetoprotein (AFP). This approach relies on a self-assembled heptamer fusion protein (A1-C4bpα), incorporating the dual functions of multivalent recognition and crosslinking a...
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| Veröffentlicht in: | Mikrochimica acta (1966) 2024-07, Vol.191 (7), p.387, Article 387 |
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| container_title | Mikrochimica acta (1966) |
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| creator | Ding, Lu Hu, Jiaqi Liu, Xing Zeng, Junyi Hu, Zhiwen Chen, Jing Zhu, Kang Duan, Hong Huang, Xiaolin |
| description | A novel construction strategy is introduced for an ultrasensitive dynamic light scattering (DLS) immunosensor targeting alpha fetoprotein (AFP). This approach relies on a self-assembled heptamer fusion protein (A1-C4bpα), incorporating the dual functions of multivalent recognition and crosslinking aggregation amplification due to the presence of seven AFP-specific A1 nanobodies on the A1-C4bpα heptamer. Leveraging antibody-functionalized magnetic nanoparticles for target AFP capture and DLS signal output, the proposed heptamer-assisted DLS immunosensor offers high sensitivity, strong specificity, and ease of operation. Under the optimized conditions, the designed DLS immunosensor demonstrates excellent linear detection of AFP in the concentration range 0.06 ng mL
−1
to 512 ng mL
−1
, with a detection limit of 15 pg mL
−1
. The selectivity, accuracy, precision, practicability, and reliability of this newly developed method were further validated through an assay of AFP levels in spiked and actual human serum samples. This work introduces a novel approach for constructing ultrasensitive DLS immunosensors, easily extendable to the sensitive determination of other targets
via
simply replacing the nanobody sequence, holding great promise in various applications, particularly in disease diagnosis.
Graphical abstract |
| doi_str_mv | 10.1007/s00604-024-06437-7 |
| format | Article |
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−1
to 512 ng mL
−1
, with a detection limit of 15 pg mL
−1
. The selectivity, accuracy, precision, practicability, and reliability of this newly developed method were further validated through an assay of AFP levels in spiked and actual human serum samples. This work introduces a novel approach for constructing ultrasensitive DLS immunosensors, easily extendable to the sensitive determination of other targets
via
simply replacing the nanobody sequence, holding great promise in various applications, particularly in disease diagnosis.
Graphical abstract</description><identifier>ISSN: 0026-3672</identifier><identifier>ISSN: 1436-5073</identifier><identifier>EISSN: 1436-5073</identifier><identifier>DOI: 10.1007/s00604-024-06437-7</identifier><identifier>PMID: 38869719</identifier><language>eng</language><publisher>Vienna: Springer Vienna</publisher><subject>alpha-Fetoproteins - analysis ; alpha-Fetoproteins - immunology ; Amplification ; Analytical Chemistry ; Antibodies, Immobilized - immunology ; Biosensing Techniques - methods ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Crosslinking ; Dynamic Light Scattering ; Humans ; Immunoassay - methods ; Immunosensors ; Limit of Detection ; Magnetite Nanoparticles - chemistry ; Microengineering ; Nanochemistry ; Nanoparticles ; Nanotechnology ; Original Paper ; Photon correlation spectroscopy ; Scattering ; Self-assembly ; Single-Domain Antibodies - chemistry ; Single-Domain Antibodies - immunology</subject><ispartof>Mikrochimica acta (1966), 2024-07, Vol.191 (7), p.387, Article 387</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c256t-8cfe9e1f2ef2addf4abf52db16ba15a5835bc0bb75b322f1f1d341850d651cf53</cites><orcidid>0000-0002-8908-0443</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00604-024-06437-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00604-024-06437-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38869719$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ding, Lu</creatorcontrib><creatorcontrib>Hu, Jiaqi</creatorcontrib><creatorcontrib>Liu, Xing</creatorcontrib><creatorcontrib>Zeng, Junyi</creatorcontrib><creatorcontrib>Hu, Zhiwen</creatorcontrib><creatorcontrib>Chen, Jing</creatorcontrib><creatorcontrib>Zhu, Kang</creatorcontrib><creatorcontrib>Duan, Hong</creatorcontrib><creatorcontrib>Huang, Xiaolin</creatorcontrib><title>Ultrasensitive dynamic light scattering immunodetection of alpha-fetoprotein using heptamer-amplified nanoparticle crosslinking aggregation</title><title>Mikrochimica acta (1966)</title><addtitle>Microchim Acta</addtitle><addtitle>Mikrochim Acta</addtitle><description>A novel construction strategy is introduced for an ultrasensitive dynamic light scattering (DLS) immunosensor targeting alpha fetoprotein (AFP). This approach relies on a self-assembled heptamer fusion protein (A1-C4bpα), incorporating the dual functions of multivalent recognition and crosslinking aggregation amplification due to the presence of seven AFP-specific A1 nanobodies on the A1-C4bpα heptamer. Leveraging antibody-functionalized magnetic nanoparticles for target AFP capture and DLS signal output, the proposed heptamer-assisted DLS immunosensor offers high sensitivity, strong specificity, and ease of operation. Under the optimized conditions, the designed DLS immunosensor demonstrates excellent linear detection of AFP in the concentration range 0.06 ng mL
−1
to 512 ng mL
−1
, with a detection limit of 15 pg mL
−1
. The selectivity, accuracy, precision, practicability, and reliability of this newly developed method were further validated through an assay of AFP levels in spiked and actual human serum samples. This work introduces a novel approach for constructing ultrasensitive DLS immunosensors, easily extendable to the sensitive determination of other targets
via
simply replacing the nanobody sequence, holding great promise in various applications, particularly in disease diagnosis.
Graphical abstract</description><subject>alpha-Fetoproteins - analysis</subject><subject>alpha-Fetoproteins - immunology</subject><subject>Amplification</subject><subject>Analytical Chemistry</subject><subject>Antibodies, Immobilized - immunology</subject><subject>Biosensing Techniques - methods</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Crosslinking</subject><subject>Dynamic Light Scattering</subject><subject>Humans</subject><subject>Immunoassay - methods</subject><subject>Immunosensors</subject><subject>Limit of Detection</subject><subject>Magnetite Nanoparticles - chemistry</subject><subject>Microengineering</subject><subject>Nanochemistry</subject><subject>Nanoparticles</subject><subject>Nanotechnology</subject><subject>Original Paper</subject><subject>Photon correlation spectroscopy</subject><subject>Scattering</subject><subject>Self-assembly</subject><subject>Single-Domain Antibodies - chemistry</subject><subject>Single-Domain Antibodies - immunology</subject><issn>0026-3672</issn><issn>1436-5073</issn><issn>1436-5073</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1u1TAQRi1ERW8LL8ACRWLDxtQ_sZ27RFVpK1ViQ9fRxBnnuiR2sB2kPgMvTdJbQGLBYjSbM9_M6BDylrOPnDFzkRnTrKZMrKVraah5QXa8lpoqZuRLsmNMaCq1EafkLOcHxrjRon5FTmXT6L3h-x35eT-WBBlD9sX_wKp_DDB5W41-OJQqWygFkw9D5adpCbHHgrb4GKroKhjnA1CHJc4pFvShWvKGHnAuMGGiMM2jdx77KkCIM6Ti7YiVTTHn0YdvGwzDkHCALfM1OXEwZnzz3M_J_eerr5c39O7L9e3lpztqhdKFNtbhHrkT6AT0vauhc0r0HdcdcAWqkaqzrOuM6qQQjjvey5o3ivVaceuUPCcfjrnr2d8XzKWdfLY4jhAwLrmVTJs9l4rJFX3_D_oQlxTW6zaq4UxryVdKHKmnzxK6dk5-gvTYctZuqtqjqnZV1T6pas069O45eukm7P-M_HazAvII5HkzgOnv7v_E_gL_QqPq</recordid><startdate>20240701</startdate><enddate>20240701</enddate><creator>Ding, Lu</creator><creator>Hu, Jiaqi</creator><creator>Liu, Xing</creator><creator>Zeng, Junyi</creator><creator>Hu, Zhiwen</creator><creator>Chen, Jing</creator><creator>Zhu, Kang</creator><creator>Duan, Hong</creator><creator>Huang, Xiaolin</creator><general>Springer Vienna</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-8908-0443</orcidid></search><sort><creationdate>20240701</creationdate><title>Ultrasensitive dynamic light scattering immunodetection of alpha-fetoprotein using heptamer-amplified nanoparticle crosslinking aggregation</title><author>Ding, Lu ; Hu, Jiaqi ; Liu, Xing ; Zeng, Junyi ; Hu, Zhiwen ; Chen, Jing ; Zhu, Kang ; Duan, Hong ; Huang, Xiaolin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c256t-8cfe9e1f2ef2addf4abf52db16ba15a5835bc0bb75b322f1f1d341850d651cf53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>alpha-Fetoproteins - analysis</topic><topic>alpha-Fetoproteins - immunology</topic><topic>Amplification</topic><topic>Analytical Chemistry</topic><topic>Antibodies, Immobilized - immunology</topic><topic>Biosensing Techniques - methods</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Crosslinking</topic><topic>Dynamic Light Scattering</topic><topic>Humans</topic><topic>Immunoassay - methods</topic><topic>Immunosensors</topic><topic>Limit of Detection</topic><topic>Magnetite Nanoparticles - chemistry</topic><topic>Microengineering</topic><topic>Nanochemistry</topic><topic>Nanoparticles</topic><topic>Nanotechnology</topic><topic>Original Paper</topic><topic>Photon correlation spectroscopy</topic><topic>Scattering</topic><topic>Self-assembly</topic><topic>Single-Domain Antibodies - chemistry</topic><topic>Single-Domain Antibodies - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ding, Lu</creatorcontrib><creatorcontrib>Hu, Jiaqi</creatorcontrib><creatorcontrib>Liu, Xing</creatorcontrib><creatorcontrib>Zeng, Junyi</creatorcontrib><creatorcontrib>Hu, Zhiwen</creatorcontrib><creatorcontrib>Chen, Jing</creatorcontrib><creatorcontrib>Zhu, Kang</creatorcontrib><creatorcontrib>Duan, Hong</creatorcontrib><creatorcontrib>Huang, Xiaolin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Mikrochimica acta (1966)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ding, Lu</au><au>Hu, Jiaqi</au><au>Liu, Xing</au><au>Zeng, Junyi</au><au>Hu, Zhiwen</au><au>Chen, Jing</au><au>Zhu, Kang</au><au>Duan, Hong</au><au>Huang, Xiaolin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ultrasensitive dynamic light scattering immunodetection of alpha-fetoprotein using heptamer-amplified nanoparticle crosslinking aggregation</atitle><jtitle>Mikrochimica acta (1966)</jtitle><stitle>Microchim Acta</stitle><addtitle>Mikrochim Acta</addtitle><date>2024-07-01</date><risdate>2024</risdate><volume>191</volume><issue>7</issue><spage>387</spage><pages>387-</pages><artnum>387</artnum><issn>0026-3672</issn><issn>1436-5073</issn><eissn>1436-5073</eissn><abstract>A novel construction strategy is introduced for an ultrasensitive dynamic light scattering (DLS) immunosensor targeting alpha fetoprotein (AFP). This approach relies on a self-assembled heptamer fusion protein (A1-C4bpα), incorporating the dual functions of multivalent recognition and crosslinking aggregation amplification due to the presence of seven AFP-specific A1 nanobodies on the A1-C4bpα heptamer. Leveraging antibody-functionalized magnetic nanoparticles for target AFP capture and DLS signal output, the proposed heptamer-assisted DLS immunosensor offers high sensitivity, strong specificity, and ease of operation. Under the optimized conditions, the designed DLS immunosensor demonstrates excellent linear detection of AFP in the concentration range 0.06 ng mL
−1
to 512 ng mL
−1
, with a detection limit of 15 pg mL
−1
. The selectivity, accuracy, precision, practicability, and reliability of this newly developed method were further validated through an assay of AFP levels in spiked and actual human serum samples. This work introduces a novel approach for constructing ultrasensitive DLS immunosensors, easily extendable to the sensitive determination of other targets
via
simply replacing the nanobody sequence, holding great promise in various applications, particularly in disease diagnosis.
Graphical abstract</abstract><cop>Vienna</cop><pub>Springer Vienna</pub><pmid>38869719</pmid><doi>10.1007/s00604-024-06437-7</doi><orcidid>https://orcid.org/0000-0002-8908-0443</orcidid></addata></record> |
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| subjects | alpha-Fetoproteins - analysis alpha-Fetoproteins - immunology Amplification Analytical Chemistry Antibodies, Immobilized - immunology Biosensing Techniques - methods Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Crosslinking Dynamic Light Scattering Humans Immunoassay - methods Immunosensors Limit of Detection Magnetite Nanoparticles - chemistry Microengineering Nanochemistry Nanoparticles Nanotechnology Original Paper Photon correlation spectroscopy Scattering Self-assembly Single-Domain Antibodies - chemistry Single-Domain Antibodies - immunology |
| title | Ultrasensitive dynamic light scattering immunodetection of alpha-fetoprotein using heptamer-amplified nanoparticle crosslinking aggregation |
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