Quantifying CO-release from a photo-CORM using 19F NMR: An investigation into light-induced CO delivery

Carbon monoxide (CO) is an innate signaling molecule that can regulate immune responses and interact with crucial elements of the circadian clock. Moreover, pharmacologically, CO has been substantiated for its therapeutic advantages in animal models of diverse pathological conditions. Given that an excessive level of CO can be toxic, it is imperative to quantify the necessary amount for therapeutic use accurately. However, estimating gaseous CO is notably challenging. Therefore, novel techniques are essential to quantify CO in therapeutic applications and overcome this obstacle precisely. The classical Myoglobin (Mb) assay technique has been extensively used to determine the amount of CO-release from CO-releasing molecules (CORMs) within therapeutic contexts. Nevertheless, specific challenges arise when applying the Mb assay to evaluate CORMs featuring innovative molecular architectures. Here, we report a fluorinated photo-CORM (CORM-FBS) for the photo-induced CO-release. We employed the 19F NMR spectroscopy approach to monitor the release of CO as well as quantitative evaluation of CO release. This new 19F NMR approach opens immense opportunities for researchers to develop reliable techniques for identifying molecular structures, quantitative studies of drug metabolism, and monitoring the reaction process. To date, the classical Myoglobin (Mb) assay has been used extensively for determining the amount and the rate of CO-release from CO-releasing molecules (CORMs) within therapeutic contexts. Nevertheless, certain challenges arise when applying the Mb assay to evaluate CORMs featuring innovative molecular architectures. Here, we report a fluorinated photo-CORM (CORM-FBS) for the photo induced CO-release. For the first time, 19F NMR is performed to quantitative analysis of CO-release from CORM-FBS under irradiation. The light induced CO-release behaviour of CORM-FBS also investigated by Myoglobin assay, time-dependent liquid-phase IR study and UV–Vis experiments. [Display omitted] •Carbon monoxide (CO) serves as an innate signaling molecule, which can regulate immune responses and interact with crucial elements of the circadian clock. To date, the classical Myoglobin (Mb) assay has been used extensively to determine the amount and rate of CO-release from CO-releasing molecules (CORMs) within therapeutic contexts.•CO release behaviour of fluorinated photo-CORM (CORM-FBS) also supported by systematic Myoglobin assay, liquid-phase IR study and UV–Vis experimen