Realization of high-performance biosensor through sandwich analysis utilizing weak value amplification

A label-free optical sandwich immunoassay sensor, utilizing weak value amplification and total internal reflection, was devised for real-time, high-sensitivity analysis and detection of low-concentration targets. 3D printed channels and sodium chloride solution were employed to ensure reproducibility, reliability, and stability of the measurements for calibration. The sandwich structure demonstrated enhanced responsiveness in the proposed optical biosensor through a comparative analysis of the direct assay and sandwich assay for detecting alpha-fetoprotein (AFP) at the same concentration. By optimizing the binding sequences of the coating antibody, target, and detection antibody in the sandwich method, a more suitable sandwich sensing approach based on weak value amplification was achieved. With this approach, the limit of detection (LOD) of 6.29 ng/mL (pM level) for AFP in PBS solution was achieved. AFP testing and regeneration experiments in human serum have proved the feasibility of our methods in detecting complex samples and the reusability of sensing chips. Additionally, the method demonstrated excellent selectivity for unpaired antigens. The efficacy of this methodology was evaluated by simultaneously detecting AFP, carcinoembryonic antigen (CEA), and CA15-3 on a singular sensor chip. In conclusion, the label-free sandwich immunoassay sensing scheme holds promise for advancing the proposed optical sensors based on weak value amplification in early diagnosis and prevention applications. Compared to other biomarker detection methods, it will be easier to promote in practical applications. A sandwich-structured biosensor based on weak-value amplification and total internal reflection is proposed to realize susceptible, label-free, and high-throughput detection of tumor markers. Compared with the direct method, it can effectively improve the detection limit and has a good application prospect in the trace detection and high-sensitivity detection of biomarkers. [Display omitted] •We proposed a weak value amplification-based biosensor for AFP detection.•The method uses a weak measurement system with total internal reflection.•The sandwich method serves as signal amplification to improve the detection limit.•The detection limit of the sandwich method for AFP is 6.29 ng/mL.•Combining with different chips can realize high throughput detection of biomarkers.