Engineering Core/Ligands Interfacial Anchors of Nanoparticles for Efficiently Inhibiting Both Aβ and Amylin Fibrillization

Accurate construction of artificial nano‐chaperones' structure is crucial for precise regulation of protein conformational transformation, facilitating effective treatment of proteopathy. However, how the ligand‐anchors of nano‐chaperones affect the spatial conformational changes in proteins remains unclear, limiting the development of efficient nano‐chaperones. In this study, three types of gold nanoparticles (AuNPs) with different core/ligands interface anchor structures (Au─NH─R, Au─S─R, and Au─C≡C─R, R = benzoic acid) are synthesized as an ideal model to investigate the effect of interfacial anchors on Aβ and amylin fibrillization. Computational results revealed that the distinct interfacial anchors imparted diverse distributions of electrostatic potential on the nanointerface and core/ligands bond strength of AuNPs, leading to differential interactions with amyloid peptides. Experimental results demonstrated that all three types of AuNPs exhibit site‐specific inhibitory effects on Aβ40 fibrillization due to preferential binding. For amylin, amino‐anchored AuNPs demonstrate strong adsorption to multiple sites on amylin and effectively inhibit fibrillization. Conversely, thiol‐ and alkyne‐anchored AuNPs adsorb at the head region of amylin, promoting folding and fibrillization. This study not only provided molecular insights into how core/ligands interfacial anchors of nanomaterials induce spatial conformational changes in amyloid peptides but also offered guidance for precisely engineering artificial‐chaperones' nanointerfaces to regulate the conformational transformation of proteins. Here, distinct core/ligands interface anchor structures (Au─NH─R, Au─S─R, and Au─C≡C─R, R = benzoic acid) are established in Au nanoparticles (AuNPs) to investigate the regulation of amyloid peptide conformation by precisely designed artificial nano‐chaperones. Interestingly, amino‐anchored AuNPs efficiently inhibit both Aβ and amylin fibrillization, whereas thiol‐ and alkyne‐anchored AuNPs only hinder Aβ misfolding but accelerate amylin fibrillization.