Influence of preconditioning on morphological and mechanical properties of human Achilles tendon in vivo

The present study aimed 1) to verify whether the effect of preconditioning was observed in the measured variables during the measurement of the human tendon in vivo (i.e., repeated contractions with breaks between trials) and 2) to determine the changes in tendon properties and their mechanisms due to submaximal repetitive contractions. Twelve healthy males participated in this study. To eliminate the effects of preconditioning, the participants rested on the measurement bed for 2 h before the start of both experiment-1 and experiment-2. In experiment-1, the measurements of elongation and hysteresis of the Achilles tendon for ramp and ballistic conditions were repeated ten times every 2 min. In experiment-2, participants performed submaximal repetitive contractions at 50 % of MVC and a frequency of 1 Hz for 10 min with a 30 s break every 2 min. Tendon mechanical properties were measured during contractions (starting 30 s and last 20 s of every 2 min), and the mean and coefficient variation (CV) of echogenicity were assessed during a 30-s rest every 2 min. In experiment-1, no significant differences in elongation and hysteresis of the tendon for ramp and ballistic contractions were found among the trials. In experiment 2, there were no significant differences in tendon elongation and hysteresis among all measurement times. Mean echogenicity increased significantly after 2 min, and CV of echogenicity decreased significantly after 4 min. These results suggest that preconditioning does not affect the elongation and hysteresis of the Achilles tendon in measuring tendon mechanical properties and submaximal repetitive contractions.