The synthesis of solid supports carrying base labile linkers to generate 3′-phosphate oligonucleotides

[Display omitted] •Oligonucleotide carrying 3′-phosphates are important molecules in biomedicine.•Solid supports functionalized with base labile linkers allow the rapid preparation of these compounds.•The 4-((2-hydroxyethyl)sulfonyl)benzamide linker provides excellent yields in the preparation of 3′-phosphorylated oligonucleotides.•Mild deprotection conditions allows the preparation of partially protected DNA fragments. Oligonucleotides carrying 3′-terminal phosphates and conjugates are important tools in molecular biology and diagnostic purposes. We described the preparation of solid supports carrying the base labile linker 4-((2-hydroxyethyl)sulfonyl)benzamide for the solid-phase synthesis of 3′-phosphorylated oligonucleotides. These supports are fully compatible with the phosphoramidite chemistry yielding the desired 3′-phosphate oligonucleotides in excellent yields. The use of mild deprotection conditions allows the generation of partially protected DNA fragments.