Effects of anti-CD20 therapy on circulating and intrathecal follicular helper T cell subsets in multiple sclerosis
Follicular helper T (Tfh) cells and their interplay with B cells likely contribute to the pathogenesis of relapsing-remitting multiple sclerosis (RRMS). Tfh cells are enriched in cerebrospinal fluid (CSF) in RRMS, but effects of anti-CD20 therapy are unknown. We investigated Tfh cells in controls, u...
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Veröffentlicht in: | Clinical immunology (Orlando, Fla.) Fla.), 2024-07, Vol.264, p.110262, Article 110262 |
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Zusammenfassung: | Follicular helper T (Tfh) cells and their interplay with B cells likely contribute to the pathogenesis of relapsing-remitting multiple sclerosis (RRMS). Tfh cells are enriched in cerebrospinal fluid (CSF) in RRMS, but effects of anti-CD20 therapy are unknown. We investigated Tfh cells in controls, untreated and anti-CD20-treated patients with RRMS using flow cytometry. CSF Tfh cells were increased in untreated patients. Compared to paired blood samples, CD25− Tfh cells were enriched in CSF in RRMS, but not in controls. Contrast-enhancing brain MRI lesions and IgG index correlated with CSF CD25− Tfh cell frequency in untreated patients with RRMS. Anti-CD20 therapy reduced the numbers of circulating PD1+ Tfh cells and CD25− Tfh cells, and the frequency of CSF CD25− Tfh cells. The study suggests that CD25− Tfh cells are recruited to the CSF in RRMS, associated with focal inflammation, and are reduced by anti-CD20 therapy.
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•CD25− Tfh cells are recruited to the cerebrospinal fluid in multiple sclerosis.•Cerebrospinal fluid CD25− Tfh cells correlate with focal inflammation and IgG index.•Anti-CD20 therapy reduces CD25− Tfh cells in blood and cerebrospinal fluid. |
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ISSN: | 1521-6616 1521-7035 1521-7035 |
DOI: | 10.1016/j.clim.2024.110262 |