$MDS and AML show elevated fractions of CD34-positive blast cell populations with a high anti-apoptotic versus proliferation ratio$

MDS and AML show elevated fractions of CD34-positive blast cell populations with a high anti-apoptotic versus proliferation ratio

This study investigates the intertwined processes of (anti-)apoptosis and cell proliferation in myelodysplastic syndromes (MDS) and acute myeloid leukemia (AML). Utilizing antibodies to Bcl-2 and Ki-67, the CD34-positive blast cell compartments in bone marrow aspirates from 50 non-malignant cases, 2...

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Veröffentlicht in:	Leukemia research 2024-07, Vol.142, p.107520, Article 107520
Hauptverfasser:	Mestrum, Stefan G.C., Roanalis, B.Y. Vanblarcum, de Wit, Norbert C.J., Drent, Roosmarie J.M., Boonen, Bert T., van Hemert, Wouter L.W., Hopman, Anton H.N., Ramaekers, Frans C.S., Leers, Math P.G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adult Aged Aged, 80 and over AML Anti-apoptosis Antigens, CD34 - analysis Antigens, CD34 - metabolism Apoptosis Bcl-2 Cell Proliferation Female Flow Cytometry Humans Ki-67 Ki-67 Antigen - analysis Ki-67 Antigen - metabolism Leukemia, Myeloid, Acute - metabolism Leukemia, Myeloid, Acute - pathology Male MDS Middle Aged Multiparameter flow cytometry Myelodysplastic Syndromes - metabolism Myelodysplastic Syndromes - pathology Proliferation Proto-Oncogene Proteins c-bcl-2 - analysis Proto-Oncogene Proteins c-bcl-2 - metabolism
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Zusammenfassung:	This study investigates the intertwined processes of (anti-)apoptosis and cell proliferation in myelodysplastic syndromes (MDS) and acute myeloid leukemia (AML). Utilizing antibodies to Bcl-2 and Ki-67, the CD34-positive blast cell compartments in bone marrow aspirates from 50 non-malignant cases, 25 MDS patients, and 25 AML patients were analyzed for their anti-apoptotic and proliferative cell fractions through ten-color flow cytometry. MDS patients exhibited a significantly increased anti-apoptotic (p=0.0014) and reduced proliferative cell fraction (p=0.0030) in their blast cell population as compared to non-malignant cases. AML patients showed an even more exacerbated trend than MDS patients. The resulting Bcl-2:Ki-67 cell fraction ratios in MDS and AML were significantly increased as compared to the non-malignant cases (p=0.0004 and p
ISSN:	0145-2126 1873-5835 1873-5835
DOI:	10.1016/j.leukres.2024.107520