Effect of continuous microwave processing on enzymes and quality attributes of bael beverage
•Continuous MW-heating showed faster enzyme inactivation (90%) than hydrothermal treatment.•Thermal sensitivity of enzymes improved under MW processing.•Structural modifications in enzyme-protein of PPO was similar for thermal and MW.•MW showed better retention of bioactive compounds and sensory att...
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Veröffentlicht in: | Food chemistry 2024-09, Vol.453, p.139621-139621, Article 139621 |
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creator | Dhar, Rishab Chakraborty, Snehasis |
description | •Continuous MW-heating showed faster enzyme inactivation (90%) than hydrothermal treatment.•Thermal sensitivity of enzymes improved under MW processing.•Structural modifications in enzyme-protein of PPO was similar for thermal and MW.•MW showed better retention of bioactive compounds and sensory attributes.•Significant colour changes were observed by both thermal and MW pasteurization.
Bael (Aegle marmelos) beverage was pasteurized using continuous-microwave (MW) and traditional thermal processing and the activity of native enzymes, pulp-hydrolyzing enzymes, bioactive, physicochemical, and sensory properties were analyzed. First-order and linear biphasic models fitted well (R2 ≥ 0.90) for enzyme inactivation and bioactive alteration kinetics, respectively. For the most resistant enzyme, polyphenoloxidase (PPO), the inactivation target of ≥ 90 % was achieved at 90 °C TMW (final temperature under MW) and 95 °C for 5 min (conventional thermal). MW treatment displayed faster enzyme inactivation and better retention of TPC and AOC. MW treatment at 90 °C TMW showed 5.3 min D-value, 90% total carotenoid content, 3.42 crisp sensory score (out of 5), and no or minor change in physicochemical attributes. Thermal and MW treatment caused the loss of 14 and 10 bioactive compounds, respectively. The secondary and tertiary structural modifications of PPO enzyme-protein revealed MW’s lethality primarily due to its thermal effects. |
doi_str_mv | 10.1016/j.foodchem.2024.139621 |
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Bael (Aegle marmelos) beverage was pasteurized using continuous-microwave (MW) and traditional thermal processing and the activity of native enzymes, pulp-hydrolyzing enzymes, bioactive, physicochemical, and sensory properties were analyzed. First-order and linear biphasic models fitted well (R2 ≥ 0.90) for enzyme inactivation and bioactive alteration kinetics, respectively. For the most resistant enzyme, polyphenoloxidase (PPO), the inactivation target of ≥ 90 % was achieved at 90 °C TMW (final temperature under MW) and 95 °C for 5 min (conventional thermal). MW treatment displayed faster enzyme inactivation and better retention of TPC and AOC. MW treatment at 90 °C TMW showed 5.3 min D-value, 90% total carotenoid content, 3.42 crisp sensory score (out of 5), and no or minor change in physicochemical attributes. Thermal and MW treatment caused the loss of 14 and 10 bioactive compounds, respectively. The secondary and tertiary structural modifications of PPO enzyme-protein revealed MW’s lethality primarily due to its thermal effects.</description><identifier>ISSN: 0308-8146</identifier><identifier>EISSN: 1873-7072</identifier><identifier>DOI: 10.1016/j.foodchem.2024.139621</identifier><identifier>PMID: 38761728</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Advanced thermal processing ; Beverages - analysis ; Catechol Oxidase - chemistry ; Catechol Oxidase - metabolism ; Circular dichroism (CD) ; Enzyme Stability ; Food Handling ; Fruit - chemistry ; Fruit - enzymology ; Fruit beverage ; Hot Temperature ; Humans ; Kinetic modeling ; Kinetics ; Liquid chromatography-mass spectroscopy (LC-MS) ; Microwaves ; Plant Proteins - chemistry ; Plant Proteins - metabolism ; Quality evaluation ; Taste</subject><ispartof>Food chemistry, 2024-09, Vol.453, p.139621-139621, Article 139621</ispartof><rights>2024 Elsevier Ltd</rights><rights>Copyright © 2024 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c368t-fe66c626de68af11dfb7632115fb9c0733a59d4817b1de669bc52946424f4c013</citedby><cites>FETCH-LOGICAL-c368t-fe66c626de68af11dfb7632115fb9c0733a59d4817b1de669bc52946424f4c013</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.foodchem.2024.139621$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38761728$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dhar, Rishab</creatorcontrib><creatorcontrib>Chakraborty, Snehasis</creatorcontrib><title>Effect of continuous microwave processing on enzymes and quality attributes of bael beverage</title><title>Food chemistry</title><addtitle>Food Chem</addtitle><description>•Continuous MW-heating showed faster enzyme inactivation (90%) than hydrothermal treatment.•Thermal sensitivity of enzymes improved under MW processing.•Structural modifications in enzyme-protein of PPO was similar for thermal and MW.•MW showed better retention of bioactive compounds and sensory attributes.•Significant colour changes were observed by both thermal and MW pasteurization.
Bael (Aegle marmelos) beverage was pasteurized using continuous-microwave (MW) and traditional thermal processing and the activity of native enzymes, pulp-hydrolyzing enzymes, bioactive, physicochemical, and sensory properties were analyzed. First-order and linear biphasic models fitted well (R2 ≥ 0.90) for enzyme inactivation and bioactive alteration kinetics, respectively. For the most resistant enzyme, polyphenoloxidase (PPO), the inactivation target of ≥ 90 % was achieved at 90 °C TMW (final temperature under MW) and 95 °C for 5 min (conventional thermal). MW treatment displayed faster enzyme inactivation and better retention of TPC and AOC. MW treatment at 90 °C TMW showed 5.3 min D-value, 90% total carotenoid content, 3.42 crisp sensory score (out of 5), and no or minor change in physicochemical attributes. Thermal and MW treatment caused the loss of 14 and 10 bioactive compounds, respectively. The secondary and tertiary structural modifications of PPO enzyme-protein revealed MW’s lethality primarily due to its thermal effects.</description><subject>Advanced thermal processing</subject><subject>Beverages - analysis</subject><subject>Catechol Oxidase - chemistry</subject><subject>Catechol Oxidase - metabolism</subject><subject>Circular dichroism (CD)</subject><subject>Enzyme Stability</subject><subject>Food Handling</subject><subject>Fruit - chemistry</subject><subject>Fruit - enzymology</subject><subject>Fruit beverage</subject><subject>Hot Temperature</subject><subject>Humans</subject><subject>Kinetic modeling</subject><subject>Kinetics</subject><subject>Liquid chromatography-mass spectroscopy (LC-MS)</subject><subject>Microwaves</subject><subject>Plant Proteins - chemistry</subject><subject>Plant Proteins - metabolism</subject><subject>Quality evaluation</subject><subject>Taste</subject><issn>0308-8146</issn><issn>1873-7072</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1P3DAQhq2Kqizb_gXkI5csHjs7cW4gBG0lpF7aWyXLccbUqyQGO1m0_fU1WuDa00ijZz7eh7FzEBsQgJe7jY-xd39o3Egh6w2oFiV8YCvQjaoa0cgTthJK6EpDjafsLOedEEIK0J_YqdINQiP1iv2-9Z7czKPnLk5zmJa4ZD4Gl-Kz3RN_TNFRzmF64HHiNP09jJS5nXr-tNghzAdu5zmFbplLuyzpLA28oz0l-0Cf2Udvh0xfXuua_bq7_Xnzrbr_8fX7zfV95RTqufKE6FBiT6itB-h916CSAFvftU40Stlt29camg4Kg23ntrKtsZa1r50AtWYXx73l26eF8mzGkB0Ng52oxDFKbBFRKlEXFI9oCZhzIm8eUxhtOhgQ5sWs2Zk3s-bFrDmaLYPnrzeWbqT-fexNZQGujgCVpPtAyWQXaHLUh1QMmz6G_934BzlEjig</recordid><startdate>20240930</startdate><enddate>20240930</enddate><creator>Dhar, Rishab</creator><creator>Chakraborty, Snehasis</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20240930</creationdate><title>Effect of continuous microwave processing on enzymes and quality attributes of bael beverage</title><author>Dhar, Rishab ; Chakraborty, Snehasis</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-fe66c626de68af11dfb7632115fb9c0733a59d4817b1de669bc52946424f4c013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Advanced thermal processing</topic><topic>Beverages - analysis</topic><topic>Catechol Oxidase - chemistry</topic><topic>Catechol Oxidase - metabolism</topic><topic>Circular dichroism (CD)</topic><topic>Enzyme Stability</topic><topic>Food Handling</topic><topic>Fruit - chemistry</topic><topic>Fruit - enzymology</topic><topic>Fruit beverage</topic><topic>Hot Temperature</topic><topic>Humans</topic><topic>Kinetic modeling</topic><topic>Kinetics</topic><topic>Liquid chromatography-mass spectroscopy (LC-MS)</topic><topic>Microwaves</topic><topic>Plant Proteins - chemistry</topic><topic>Plant Proteins - metabolism</topic><topic>Quality evaluation</topic><topic>Taste</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dhar, Rishab</creatorcontrib><creatorcontrib>Chakraborty, Snehasis</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dhar, Rishab</au><au>Chakraborty, Snehasis</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of continuous microwave processing on enzymes and quality attributes of bael beverage</atitle><jtitle>Food chemistry</jtitle><addtitle>Food Chem</addtitle><date>2024-09-30</date><risdate>2024</risdate><volume>453</volume><spage>139621</spage><epage>139621</epage><pages>139621-139621</pages><artnum>139621</artnum><issn>0308-8146</issn><eissn>1873-7072</eissn><abstract>•Continuous MW-heating showed faster enzyme inactivation (90%) than hydrothermal treatment.•Thermal sensitivity of enzymes improved under MW processing.•Structural modifications in enzyme-protein of PPO was similar for thermal and MW.•MW showed better retention of bioactive compounds and sensory attributes.•Significant colour changes were observed by both thermal and MW pasteurization.
Bael (Aegle marmelos) beverage was pasteurized using continuous-microwave (MW) and traditional thermal processing and the activity of native enzymes, pulp-hydrolyzing enzymes, bioactive, physicochemical, and sensory properties were analyzed. First-order and linear biphasic models fitted well (R2 ≥ 0.90) for enzyme inactivation and bioactive alteration kinetics, respectively. For the most resistant enzyme, polyphenoloxidase (PPO), the inactivation target of ≥ 90 % was achieved at 90 °C TMW (final temperature under MW) and 95 °C for 5 min (conventional thermal). MW treatment displayed faster enzyme inactivation and better retention of TPC and AOC. MW treatment at 90 °C TMW showed 5.3 min D-value, 90% total carotenoid content, 3.42 crisp sensory score (out of 5), and no or minor change in physicochemical attributes. Thermal and MW treatment caused the loss of 14 and 10 bioactive compounds, respectively. The secondary and tertiary structural modifications of PPO enzyme-protein revealed MW’s lethality primarily due to its thermal effects.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>38761728</pmid><doi>10.1016/j.foodchem.2024.139621</doi><tpages>1</tpages></addata></record> |
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subjects | Advanced thermal processing Beverages - analysis Catechol Oxidase - chemistry Catechol Oxidase - metabolism Circular dichroism (CD) Enzyme Stability Food Handling Fruit - chemistry Fruit - enzymology Fruit beverage Hot Temperature Humans Kinetic modeling Kinetics Liquid chromatography-mass spectroscopy (LC-MS) Microwaves Plant Proteins - chemistry Plant Proteins - metabolism Quality evaluation Taste |
title | Effect of continuous microwave processing on enzymes and quality attributes of bael beverage |
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