Effect of continuous microwave processing on enzymes and quality attributes of bael beverage

•Continuous MW-heating showed faster enzyme inactivation (90%) than hydrothermal treatment.•Thermal sensitivity of enzymes improved under MW processing.•Structural modifications in enzyme-protein of PPO was similar for thermal and MW.•MW showed better retention of bioactive compounds and sensory att...

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Veröffentlicht in:Food chemistry 2024-09, Vol.453, p.139621-139621, Article 139621
Hauptverfasser: Dhar, Rishab, Chakraborty, Snehasis
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description •Continuous MW-heating showed faster enzyme inactivation (90%) than hydrothermal treatment.•Thermal sensitivity of enzymes improved under MW processing.•Structural modifications in enzyme-protein of PPO was similar for thermal and MW.•MW showed better retention of bioactive compounds and sensory attributes.•Significant colour changes were observed by both thermal and MW pasteurization. Bael (Aegle marmelos) beverage was pasteurized using continuous-microwave (MW) and traditional thermal processing and the activity of native enzymes, pulp-hydrolyzing enzymes, bioactive, physicochemical, and sensory properties were analyzed. First-order and linear biphasic models fitted well (R2 ≥ 0.90) for enzyme inactivation and bioactive alteration kinetics, respectively. For the most resistant enzyme, polyphenoloxidase (PPO), the inactivation target of ≥ 90 % was achieved at 90 °C TMW (final temperature under MW) and 95 °C for 5 min (conventional thermal). MW treatment displayed faster enzyme inactivation and better retention of TPC and AOC. MW treatment at 90 °C TMW showed 5.3 min D-value, 90% total carotenoid content, 3.42 crisp sensory score (out of 5), and no or minor change in physicochemical attributes. Thermal and MW treatment caused the loss of 14 and 10 bioactive compounds, respectively. The secondary and tertiary structural modifications of PPO enzyme-protein revealed MW’s lethality primarily due to its thermal effects.
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Bael (Aegle marmelos) beverage was pasteurized using continuous-microwave (MW) and traditional thermal processing and the activity of native enzymes, pulp-hydrolyzing enzymes, bioactive, physicochemical, and sensory properties were analyzed. First-order and linear biphasic models fitted well (R2 ≥ 0.90) for enzyme inactivation and bioactive alteration kinetics, respectively. For the most resistant enzyme, polyphenoloxidase (PPO), the inactivation target of ≥ 90 % was achieved at 90 °C TMW (final temperature under MW) and 95 °C for 5 min (conventional thermal). MW treatment displayed faster enzyme inactivation and better retention of TPC and AOC. MW treatment at 90 °C TMW showed 5.3 min D-value, 90% total carotenoid content, 3.42 crisp sensory score (out of 5), and no or minor change in physicochemical attributes. Thermal and MW treatment caused the loss of 14 and 10 bioactive compounds, respectively. 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Bael (Aegle marmelos) beverage was pasteurized using continuous-microwave (MW) and traditional thermal processing and the activity of native enzymes, pulp-hydrolyzing enzymes, bioactive, physicochemical, and sensory properties were analyzed. First-order and linear biphasic models fitted well (R2 ≥ 0.90) for enzyme inactivation and bioactive alteration kinetics, respectively. For the most resistant enzyme, polyphenoloxidase (PPO), the inactivation target of ≥ 90 % was achieved at 90 °C TMW (final temperature under MW) and 95 °C for 5 min (conventional thermal). MW treatment displayed faster enzyme inactivation and better retention of TPC and AOC. MW treatment at 90 °C TMW showed 5.3 min D-value, 90% total carotenoid content, 3.42 crisp sensory score (out of 5), and no or minor change in physicochemical attributes. Thermal and MW treatment caused the loss of 14 and 10 bioactive compounds, respectively. 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source MEDLINE; ScienceDirect Journals (5 years ago - present)
subjects Advanced thermal processing
Beverages - analysis
Catechol Oxidase - chemistry
Catechol Oxidase - metabolism
Circular dichroism (CD)
Enzyme Stability
Food Handling
Fruit - chemistry
Fruit - enzymology
Fruit beverage
Hot Temperature
Humans
Kinetic modeling
Kinetics
Liquid chromatography-mass spectroscopy (LC-MS)
Microwaves
Plant Proteins - chemistry
Plant Proteins - metabolism
Quality evaluation
Taste
title Effect of continuous microwave processing on enzymes and quality attributes of bael beverage
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