Bead‐beating assay during synovial fluid DNA extraction improves real‐time PCR accuracy for periprosthetic joint infection

Polymerase chain reaction (PCR)‐based genetic diagnosis is a rapid and sensitive method to diagnose periprosthetic joint infection (PJI). DNA extraction using bead beating is an effective method for collecting bacterial genes in Gram‐positive bacteria. We compared the detection accuracy between the conventional and bead‐beating DNA extraction assay. The detection rate improved from 86.7% using the conventional method to 95.6% using the bead‐beating. Our results suggest that bead‐beating during DNA extraction can improve the accuracy of PCR‐based genetic diagnosis of PJI.