Isolation and characterization of two Staphylococcus aureus lytic bacteriophages “Huma” and “Simurgh”

Nowadays finding the new antimicrobials is necessary due to the emerging of multidrug resistant strains. The present study aimed to isolate and characterize bacteriophages against S. aureus. Strains Huma and Simurgh were the two podovirus morphology phages which isolated and then characterized. Huma...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Virology (New York, N.Y.) N.Y.), 2024-07, Vol.595, p.110090-110090, Article 110090
Hauptverfasser: Sharifi, Fatemeh, Montaseri, Maryam, Yousefi, Mohammad Hashem, Shekarforoush, Seyed Shahram, Berizi, Enayat, Wagemans, Jeroen, Vallino, Marta, Hosseinzadeh, Saeid
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Nowadays finding the new antimicrobials is necessary due to the emerging of multidrug resistant strains. The present study aimed to isolate and characterize bacteriophages against S. aureus. Strains Huma and Simurgh were the two podovirus morphology phages which isolated and then characterized. Huma and Simurgh had a genome size of 16,853 and 17,245 bp, respectively and both were Rosenblumvirus with G + C content of 29%. No lysogeny-related genes, nor virulence genes were identified in their genomes. They were lytic only against two out of four S. aureus strains. They also were able to inhibit S. aureus for 8 h in-vitro. Both showed a rapid adsorption. Huma and Simurgh had the latent period of 80 and 60 m and the burst sizes of 45 and 40 PFU/ml and also, they showed very low cell toxicity of 1.23%–1.79% on HT-29 cells, respectively. Thus, they can be considered potential candidates for biocontrol applications. •Huma & Simurgh had genome sizes of 16,853 and 17,245 bp and GC content of 29%.•No lysogeny-related, nor virulence genes were identified in their genomes.•They had latent period of 80 and 60 min and burst sizes of 45 and 40 PFU/ml.
ISSN:0042-6822
1096-0341
DOI:10.1016/j.virol.2024.110090