In situ monitoring of loading and elution processes of cryoprotectants at the single-cell level with Raman spectroscopy
The analysis of cell membrane permeability plays a crucial role in improving the procedures of cell cryopreservation, which will affect the specific parameter settings in loading, removal and cooling processes. However, existing studies have mostly focused on deriving permeability parameters through...
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Veröffentlicht in: | Analytica chimica acta 2024-06, Vol.1307, p.342640-342640, Article 342640 |
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Sprache: | eng |
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Zusammenfassung: | The analysis of cell membrane permeability plays a crucial role in improving the procedures of cell cryopreservation, which will affect the specific parameter settings in loading, removal and cooling processes. However, existing studies have mostly focused on deriving permeability parameters through osmotic theoretical models and cell volume response analysis, and there is still a lack of the direct experimental evidence and analysis at the single-cell level regarding the migration of cryoprotectants.
In this work, a side perfusion microfluidics chips combined with Raman spectroscopy system was built to monitor in situ the Raman spectroscopy of extracellular and intracellular solution during loading and elution process with different cryoprotectant solution systems (single and dual component). And it was found that loading a high concentration cryoprotectant solution system through a single elution cycle may result in significant residual protective agent, which can be mitigated by employing a multi-component formula but multiple elution operations are still necessary. Furthermore, the collected spectral signals were marked and analyzed to was perform preliminary relative quantitative analysis. The results showed that the intracellular concentration changes can be accurately quantified by the Raman spectrum and are closely related to the extracellular solution concentration changes.
By using the method of small flow perfusion (≤20 μL/min) in the side microfluidic chip after the gravity sedimentation of cells, the continuous loading and elution process of different cryoprotectants on chip and the spectral acquisition can be realized. The intracellular and extracellular concentrations can be quantified in situ based on the ratio of spectral peak intensities. These results indicate that spectroscopic analysis can be used to effectively monitor intracellular cryoprotectant residues.
Dynamic evaluation of cell permeability by combining Raman spectroscopy and Microfluidics technology. [Display omitted]
•In-situ non-destructive monitoring of the cryoprotectant loading and elution process by Raman spectroscopy.•Loading and elution the cryoprotectants at low perfusion flow rates after cell gravity deposition.•The spectral curve reveals the residue of the cryoprotectant after a single elution inside the cell sample. |
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ISSN: | 0003-2670 1873-4324 |
DOI: | 10.1016/j.aca.2024.342640 |