Understanding the nature of blast resistance in combined bacterial leaf blight and blast gene pyramided lines of rice variety tellahamsa

Background Rice blast and bacterial leaf blight (BLB) are the most limiting factors for rice production in the world which cause yield losses typically ranging from 20 to 30% and can be as high as 50% in some areas of Asia especially India under severe infection conditions. Methods and results An improved line of Tellahamsa, TH-625-491 having two BLB resistance genes ( xa13 and Xa21 ) and two blast resistance genes ( Pi54 and Pi1 ) with 95% Tellahamsa genome was used in the present study. TH-625-491 was validated for all four target genes and was used for backcrossing with Tellahamsa. Seventeen IBC 1 F 1 plants heterozygous for all four target genes, 19 IBC 1 F 2 plants homozygous for four, three and two gene combinations and 19 IBC 1 F 2:3 plants also homozygous for four, three and two gene combinations were observed. Among seventeen IBC 1 F 1 plants, IBC 1 F 1 -62 plant recorded highest recurrent parent genome (97.5%) covering 75 polymorphic markers. Out of the total of 920 IBC 1 F 2 plants screened, 19 homozygous plants were homozygous for four, three and two target genes along with bacterial blight resistance. Background analysis was done in all 19 homozygous IBC 1 F 2 plants possessing BLB resistance (possessing xa13 , Xa21, Pi54 and Pi1 in different combinations) with five parental polymorphic SSR markers. IBC 1 F 2 -62-515 recovered 98.5% recurrent parent genome. The four, three and two gene pyramided lines of Tellahamsa exhibited varying resistance to blast. Conclusions Results show that there might be presence of antagonistic effect between bacterial blight and blast resistance genes since the lines with Pi54 and Pi1 combination are showing better resistance than the combinations with both bacterial blight and blast resistance genes.