Candida albicans cDNA library screening reveals novel potential diagnostic targets for invasive candidiasis
•Candida albicans germ tube antibodies disclose invasive candidiasis biomarkers•The cytoplasmic coatomer subunit gamma can be a biomarker for invasive candidiasis•Specific antibodies of invasive candidiasis target a Candida metallo-aminopeptidase•Diagnostic potential of a hyphally regulated cell wal...
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Veröffentlicht in: | Diagnostic microbiology and infectious disease 2024-07, Vol.109 (3), p.116311-116311, Article 116311 |
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Sprache: | eng |
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Zusammenfassung: | •Candida albicans germ tube antibodies disclose invasive candidiasis biomarkers•The cytoplasmic coatomer subunit gamma can be a biomarker for invasive candidiasis•Specific antibodies of invasive candidiasis target a Candida metallo-aminopeptidase•Diagnostic potential of a hyphally regulated cell wall protein 1 subterminal region
The detection of patterns associated with the invasive form of Candida albicans, such as Candida albicans germ tube antibodies (CAGTA), is a useful complement to blood culture for Invasive Candidiasis (IC) diagnosis. As CAGTA are detected by a non-standardisable and non-automatable technique, a Candida albicans cDNA expression library was screened with CAGTA isolated from serum of an animal model of invasive candidiasis, and five protein targets were identified: hyphally regulated cell wall protein 1 (Hyr1), enolase 1 (Eno1), coatomer subunit gamma (Sec21), a metallo-aminopeptidase (Ape2) and cystathionine gamma-lyase (Cys3). Homology with proteins from other organisms rules out Cys3 as a good biomarker while Sec21 results suggest that it is not in the germ tubes surface but secreted to the external environment. Our analysis propose Ape2, Sec21 and a region of Hyr1 different from the one currently being studied for immunoprotection as potential biomarker candidates for the diagnosis of IC. |
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ISSN: | 0732-8893 1879-0070 |
DOI: | 10.1016/j.diagmicrobio.2024.116311 |