Immune profiles of MCP-1 with M tb antigens and recombinant cytokines stimulation in tuberculosis

•The household contacts of active TB patients are at higher risk of progressing from latent infection to active TB.•Understanding the interactions between M tb antigens, recombinant cytokines, and chemokines offers strategies for TB control.•MCP-1 secretion and expression reveals distinct patterns in active TB and their household contactsduring TB infection.•Our study demonstrates the predictive value of MCP-1 levels in identifying high risk individuals towards TB. Tuberculosis is caused by Mycobacterium tuberculosis (M tb), which is recognized by macrophages and produces inflammatory cytokines, and chemokines at the site of infection. The present study was proposed to understand the interaction of M tb antigens, cytokines, and chemokines. We have evaluated the chemokine MCP-1 levels and its expression in PBMCs stimulated with M tb antigens Ag85A, ESAT6 and recombinant cytokines rhTNF-α, rhIFN-γ, rhTGF-β, and rhIL-10 in active pulmonary TB (APTB) patients, household contacts (HHC) at 0 months, 6 months and healthy controls (HC). We have observed low levels of MCP-1 with Ag85A, ESAT6, and rhTNF-α stimulations in APTB 0M compared to HHC and HC (p