Unraveling the immune response of the spleen in sepsis using green‐synthesized silver nanoparticles from pomegranate peel extracts
Sepsis is a serious disease characterized by an inappropriate host response to infection, resulting in widespread inflammation and systemic organ failure. The aim of this research is to investigate the possibility of pomegranate peel‐derived silver nanoparticles (PGNP) as a potential alternative the...
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| Veröffentlicht in: | Microscopy research and technique 2024-09, Vol.87 (9), p.2034-2042 |
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| creator | Marey, Amal M. Dkhil, Mohamed A. Abdel‐Moneim, Ahmed E. Santourlidis, Simeon Abdel‐Gaber, Rewaida Abdalla, Mohga S. |
| description | Sepsis is a serious disease characterized by an inappropriate host response to infection, resulting in widespread inflammation and systemic organ failure. The aim of this research is to investigate the possibility of pomegranate peel‐derived silver nanoparticles (PGNP) as a potential alternative therapy for sepsis. Characterization using transmission electron microscopy revealed 10–30 nm spherical nanoparticles. In a rat model of sepsis, PGNP treatment improved spleen health, histology, and immune response as compared with septic rats. In rats treated with PGNP during sepsis, significant alterations in oxidative stress markers (p |
| doi_str_mv | 10.1002/jemt.24575 |
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Research Highlights
The study explored the potential medicinal benefits of pomegranate peel‐derived silver nanoparticles (PGNP) in the treatment of sepsis.
PGNP suppressed pro‐inflammatory cytokines and enhanced the immune response.
The study recommends PGNP as a viable substitute treatment.</description><identifier>ISSN: 1059-910X</identifier><identifier>ISSN: 1097-0029</identifier><identifier>EISSN: 1097-0029</identifier><identifier>DOI: 10.1002/jemt.24575</identifier><identifier>PMID: 38634191</identifier><language>eng</language><publisher>Hoboken, USA: John Wiley & Sons, Inc</publisher><subject>Antibiotic resistance ; Apoptosis ; BAX protein ; Gene expression ; Glutathione ; Histology ; Immune response ; Immune system ; Immunology ; Impact resistance ; Inflammation ; Nanoparticles ; nanosilver ; Nitric oxide ; Oxidative stress ; Plant extracts ; pomegranate ; Proteins ; rats ; Sepsis ; Silver ; Spleen ; spleen response ; Superoxide dismutase ; Transmission electron microscopy</subject><ispartof>Microscopy research and technique, 2024-09, Vol.87 (9), p.2034-2042</ispartof><rights>2024 Wiley Periodicals LLC.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3165-fbe81cbc41c193ad1bd02d471c0a33cd75bccf2aa40ab3d72da242e200280a6d3</cites><orcidid>0000-0001-9263-6871 ; 0000-0003-1869-5800</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjemt.24575$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjemt.24575$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38634191$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Marey, Amal M.</creatorcontrib><creatorcontrib>Dkhil, Mohamed A.</creatorcontrib><creatorcontrib>Abdel‐Moneim, Ahmed E.</creatorcontrib><creatorcontrib>Santourlidis, Simeon</creatorcontrib><creatorcontrib>Abdel‐Gaber, Rewaida</creatorcontrib><creatorcontrib>Abdalla, Mohga S.</creatorcontrib><title>Unraveling the immune response of the spleen in sepsis using green‐synthesized silver nanoparticles from pomegranate peel extracts</title><title>Microscopy research and technique</title><addtitle>Microsc Res Tech</addtitle><description>Sepsis is a serious disease characterized by an inappropriate host response to infection, resulting in widespread inflammation and systemic organ failure. The aim of this research is to investigate the possibility of pomegranate peel‐derived silver nanoparticles (PGNP) as a potential alternative therapy for sepsis. Characterization using transmission electron microscopy revealed 10–30 nm spherical nanoparticles. In a rat model of sepsis, PGNP treatment improved spleen health, histology, and immune response as compared with septic rats. In rats treated with PGNP during sepsis, significant alterations in oxidative stress markers (p < .01) were observed. These included elevated levels of glutathione (0.63 ± 0.08 mmol/mg protein), reduced concentrations of nitric oxide (8.7 ± 0.8 μ mol/mg protein) and malondialdehyde (2.2 ± 0.3 nmol/mg protein), as well as increased activity of superoxide dismutase (159 ± 33 U/mg protein). Following PGNP administration, gene expression analysis revealed a decrease in spleen IL‐1β, IL‐6, and TNF‐α, highlighting its anti‐inflammatory potential. Furthermore, PGNP effectively controlled apoptosis‐related genes (Bax, Bcl‐2, and Casp3), indicating its role in cellular survival pathways. This study sheds light on the immunological regulation of the spleen during sepsis using PGNP, demonstrating its potential as a new effective treatment approach. The study emphasizes the necessity of continuing to investigate and develop alternative medicines, particularly in light of antibiotic resistance and the global impact of sepsis.
Research Highlights
The study explored the potential medicinal benefits of pomegranate peel‐derived silver nanoparticles (PGNP) in the treatment of sepsis.
PGNP suppressed pro‐inflammatory cytokines and enhanced the immune response.
The study recommends PGNP as a viable substitute treatment.</description><subject>Antibiotic resistance</subject><subject>Apoptosis</subject><subject>BAX protein</subject><subject>Gene expression</subject><subject>Glutathione</subject><subject>Histology</subject><subject>Immune response</subject><subject>Immune system</subject><subject>Immunology</subject><subject>Impact resistance</subject><subject>Inflammation</subject><subject>Nanoparticles</subject><subject>nanosilver</subject><subject>Nitric oxide</subject><subject>Oxidative stress</subject><subject>Plant extracts</subject><subject>pomegranate</subject><subject>Proteins</subject><subject>rats</subject><subject>Sepsis</subject><subject>Silver</subject><subject>Spleen</subject><subject>spleen response</subject><subject>Superoxide dismutase</subject><subject>Transmission electron microscopy</subject><issn>1059-910X</issn><issn>1097-0029</issn><issn>1097-0029</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp90cFu1DAQBuAIgWgpXHgAZIkLQkrriZ1NfERVgVZFvbQSt8ixJ4tXjh08SWE5ceABeEaeBG-3cODAydbo8y9r_qJ4DvwYOK9ONjjOx5Wsm_pBcQhcNWWeqoe7e61KBfzjQfGEaMM5QA3ycXEg2pWQoOCw-HETkr5F78KazZ-QuXFcArKENMVAyOJwN6bJIwbmAiOcyBFbaPdinfL01_eftA1ZkfuGlpHzt5hY0CFOOs3OeCQ2pDiyKY64TjroGdmE6Bl-nZM2Mz0tHg3aEz67P4-Km7dn16fvy8urd-enby5LI2BVl0OPLZjeSDCghLbQW15Z2YDhWghjm7o3Zqi0llz3wjaV1ZWssMrbaLleWXFUvNrnTil-XpDmbnRk0HsdMC7UCS6hEiCVzPTlP3QTlxTy77JqV03bKKmyer1XJkWihEM3JTfqtO2Ad7tuul033V03Gb-4j1z6Ee1f-qeMDGAPvjiP2_9EdRdnH673ob8BQsqeIA</recordid><startdate>202409</startdate><enddate>202409</enddate><creator>Marey, Amal M.</creator><creator>Dkhil, Mohamed A.</creator><creator>Abdel‐Moneim, Ahmed E.</creator><creator>Santourlidis, Simeon</creator><creator>Abdel‐Gaber, Rewaida</creator><creator>Abdalla, Mohga S.</creator><general>John Wiley & Sons, Inc</general><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QP</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7SS</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>7U7</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-9263-6871</orcidid><orcidid>https://orcid.org/0000-0003-1869-5800</orcidid></search><sort><creationdate>202409</creationdate><title>Unraveling the immune response of the spleen in sepsis using green‐synthesized silver nanoparticles from pomegranate peel extracts</title><author>Marey, Amal M. ; Dkhil, Mohamed A. ; Abdel‐Moneim, Ahmed E. ; Santourlidis, Simeon ; Abdel‐Gaber, Rewaida ; Abdalla, Mohga S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3165-fbe81cbc41c193ad1bd02d471c0a33cd75bccf2aa40ab3d72da242e200280a6d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Antibiotic resistance</topic><topic>Apoptosis</topic><topic>BAX protein</topic><topic>Gene expression</topic><topic>Glutathione</topic><topic>Histology</topic><topic>Immune response</topic><topic>Immune system</topic><topic>Immunology</topic><topic>Impact resistance</topic><topic>Inflammation</topic><topic>Nanoparticles</topic><topic>nanosilver</topic><topic>Nitric oxide</topic><topic>Oxidative stress</topic><topic>Plant extracts</topic><topic>pomegranate</topic><topic>Proteins</topic><topic>rats</topic><topic>Sepsis</topic><topic>Silver</topic><topic>Spleen</topic><topic>spleen response</topic><topic>Superoxide dismutase</topic><topic>Transmission electron microscopy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Marey, Amal M.</creatorcontrib><creatorcontrib>Dkhil, Mohamed A.</creatorcontrib><creatorcontrib>Abdel‐Moneim, Ahmed E.</creatorcontrib><creatorcontrib>Santourlidis, Simeon</creatorcontrib><creatorcontrib>Abdel‐Gaber, Rewaida</creatorcontrib><creatorcontrib>Abdalla, Mohga S.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Microscopy research and technique</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Marey, Amal M.</au><au>Dkhil, Mohamed A.</au><au>Abdel‐Moneim, Ahmed E.</au><au>Santourlidis, Simeon</au><au>Abdel‐Gaber, Rewaida</au><au>Abdalla, Mohga S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Unraveling the immune response of the spleen in sepsis using green‐synthesized silver nanoparticles from pomegranate peel extracts</atitle><jtitle>Microscopy research and technique</jtitle><addtitle>Microsc Res Tech</addtitle><date>2024-09</date><risdate>2024</risdate><volume>87</volume><issue>9</issue><spage>2034</spage><epage>2042</epage><pages>2034-2042</pages><issn>1059-910X</issn><issn>1097-0029</issn><eissn>1097-0029</eissn><abstract>Sepsis is a serious disease characterized by an inappropriate host response to infection, resulting in widespread inflammation and systemic organ failure. The aim of this research is to investigate the possibility of pomegranate peel‐derived silver nanoparticles (PGNP) as a potential alternative therapy for sepsis. Characterization using transmission electron microscopy revealed 10–30 nm spherical nanoparticles. In a rat model of sepsis, PGNP treatment improved spleen health, histology, and immune response as compared with septic rats. In rats treated with PGNP during sepsis, significant alterations in oxidative stress markers (p < .01) were observed. These included elevated levels of glutathione (0.63 ± 0.08 mmol/mg protein), reduced concentrations of nitric oxide (8.7 ± 0.8 μ mol/mg protein) and malondialdehyde (2.2 ± 0.3 nmol/mg protein), as well as increased activity of superoxide dismutase (159 ± 33 U/mg protein). Following PGNP administration, gene expression analysis revealed a decrease in spleen IL‐1β, IL‐6, and TNF‐α, highlighting its anti‐inflammatory potential. Furthermore, PGNP effectively controlled apoptosis‐related genes (Bax, Bcl‐2, and Casp3), indicating its role in cellular survival pathways. This study sheds light on the immunological regulation of the spleen during sepsis using PGNP, demonstrating its potential as a new effective treatment approach. The study emphasizes the necessity of continuing to investigate and develop alternative medicines, particularly in light of antibiotic resistance and the global impact of sepsis.
Research Highlights
The study explored the potential medicinal benefits of pomegranate peel‐derived silver nanoparticles (PGNP) in the treatment of sepsis.
PGNP suppressed pro‐inflammatory cytokines and enhanced the immune response.
The study recommends PGNP as a viable substitute treatment.</abstract><cop>Hoboken, USA</cop><pub>John Wiley & Sons, Inc</pub><pmid>38634191</pmid><doi>10.1002/jemt.24575</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-9263-6871</orcidid><orcidid>https://orcid.org/0000-0003-1869-5800</orcidid></addata></record> |
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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | Antibiotic resistance Apoptosis BAX protein Gene expression Glutathione Histology Immune response Immune system Immunology Impact resistance Inflammation Nanoparticles nanosilver Nitric oxide Oxidative stress Plant extracts pomegranate Proteins rats Sepsis Silver Spleen spleen response Superoxide dismutase Transmission electron microscopy |
| title | Unraveling the immune response of the spleen in sepsis using green‐synthesized silver nanoparticles from pomegranate peel extracts |
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