A pilot-scale study on the removal of binary mixture (ciprofloxacin and norfloxacin) by Scenedesmus obliquus: Optimization, biotransformation, and biofuel profile

Ciprofloxacin (CIP) and norfloxacin (NOR) belong to the organic contaminants of emerging concern (OCECs) that are frequently detected in wastewater matrices at ng/L to mg/L concentrations. This study investigates the potential of Scenedesmus obliquus in the treatment of CIP and NOR as a binary mixture from raw wastewater. Optimization of inoculum was done to find the required cell density concentration that has less inhibition and high removal. The optimum inoculum (cell density: 200 × 105 cells/mL and OD680: 1.0) has shown 75% removal with no inhibition of growth. A pilot scale study was conducted in controlled environment using high-rate algal pond to investigate the contribution of abiotic and biotic removal. Abiotic removal is negligible in comparison with the biotic contribution of removal. The order of removal efficiency is observed as COD (88%) > NOR (84.8%) > CIP (84.6%) > NH4+ (71.7%) with biodegradation as the major removal mechanism. Biotransformed products of CIP + NOR were identified inside the Scenedesmus obliquus. During the pilot-scale study, Biomass (3.70 ± 0.07 g/L) was harvested with carbohydrates (17.85 ± 0.1%), lipids (38.36 ± 0.13%), and proteins (28.18 ± 1.63%). Lipid productivity in binary mixture was 2.6 times higher than the lipid production in control condition. Transesterification of these lipids yielded good biofuel composition of 32.72% of saturated fatty acids and 21.7% of unsaturated fatty acids. [Display omitted] •Optimization of inoculum was done to increase the adaptability and removal of CIP + NOR.•84% of antibiotics (CIP + NOR) were removed from the HRAP pilot plant.•Biodegradation was the major removal mechanism identified.•Biotransformed products were identified inside the Scenedesmus obliquus.•FAME assessment showed good biofuel properties in biodiesel.