Unequal carbon and nitrogen translocation between ramets affects sexual reproductive performance of the clonal grass Leymus chinensis under nitrogen addition

Sexual reproduction is crucial for population continuity in clonal plants. The effect of nutrient translocation between ramets on sexual reproduction of clonal plants under nitrogen addition remains unclear. In this study, we focused on clonal fragments of Leymus chinensis reproductive ramets with different number of vegetative ramets connected to tillering nodes. A series of pot experiments was conducted under nitrogen addition, including 13C and 15N bidirectional labelling of vegetative ramets and reproductive ramets at the milk-ripe stage, determination of the 13C and 15N amount translocated, and assessment of the quantitative characteristics, nitrogen and carbon concentrations of reproductive ramets and vegetative ramets. Nitrogen addition promoted the translocation of 13C while inhibiting 15N between vegetative ramets and reproductive ramets. With an increase in the number of connected vegetative ramets, the 13C translocated by reproductive ramets and the 15N translocated by reproductive and vegetative ramets gradually increased. The translocation of 13C and 15N between vegetative and reproductive ramets was bidirectional and unequal. The translocated amount of 13C and 15N from reproductive ramets to vegetative ramets was always higher than that from vegetative ramets to reproductive ramets. Nitrogen addition did not prominently affect the sexual reproductive performance of L. chinensis, whereas the number of connected vegetative ramets both positively and negatively affected sexual reproductive performance. Ramet biomass is an important driver of nutrient acquisition by L. chinensis ramets. We demonstrate for the first time that unequal nutrient translocation between ramets affects sexual reproductive performance in L. chinensis. The findings contribute to an enhanced understanding of the reproductive strategies of clonal plant populations in future environments. [Display omitted] •Sexual reproduction is vital to clonal plant population recruitment and adaptation.•Nitrogen addition promoted the translocation of 13C while inhibiting 15N between ramets.•The translocation of 13C and 15N between ramets was bidirectional and unequal.•The net 13C and 15N flowed to vegetative ramets and ramet biomass is an important driving factor.•Unequal nutrient translocation between ramets help explain the low sexual reproduction in L. chinensis.