Polyzwitterionic micelles with antimicrobial-conjugation for eradication of drug-resistant bacterial biofilms

The presence of bacterial biofilms has presented a significant challenge to human health. This study presents the development of biofilm microenvironment-responsive polymeric micelles as a novel approach to address the challenges posed by bacterial biofilms. These micelles are composed of two key components: a zwitterionic component, inspired by protein isoelectric points, containing balanced quantities of primary amines and carboxylic groups that undergo a positive charge transformation in acidic microenvironments, and a hydrophobic triclosan conjugate capable of releasing triclosan in the presence of bacterial lipases. Through the synergistic combination of pH-responsiveness and lipase-responsiveness, we have significantly improved drug penetration into biofilms and enhanced its efficacy in killing bacteria. With their remarkable drug-loading capacity and the ability to specifically target and eliminate bacteria within biofilms, these zwitterionic polymeric micelles hold great promise as an effective alternative for treating biofilm-associated infections. Their unique properties enable efficient drug delivery and heightened effectiveness against biofilm-related infections. [Display omitted] •Microenvironment-responsive micelles address bacterial biofilm challenges.•Polymeric micelles have zwitterionic component and hydrophobic triclosan conjugate.•pH and lipase responsiveness improve drug penetration, enhancing bacterial killing.•Micelles target bacteria in biofilms, ensuring efficient drug delivery.