Discovery of novel amide derivatives as potent quorum sensing inhibitors of Pseudomonas aeruginosa

With the increasing reports of antibiotic resistance in this species, Pseudomonas aeruginosa is a common human pathogen with important implications for public health. Bacterial quorum sensing (QS) systems are potentially broad and versatile targets for developing new antimicrobial compounds. While previous reports have demonstrated that certain amide compounds can inhibit bacterial growth, there are few reports on the specific inhibitory effects of these compounds on bacterial quorum sensing systems. In this study, thirty-one amide derivatives were synthesized. The results of the biological activity assessment indicated that A9 and B6 could significantly inhibit the expression of lasB, rhlA, and pqsA, effectively reducing several virulence factors regulated by the QS systems of PAO1. Additionally, compound A9 attenuated the pathogenicity of PAO1 to Galleria mellonella larvae. Meanwhile, RT-qPCR, SPR, and molecular docking studies were conducted to explore the mechanism of these compounds, which suggests that compound A9 inhibited the QS systems by binding with LasR and PqsR, especially PqsR. In conclusion, amide derivatives A9 and B6 exhibit promising potential for further development as novel QS inhibitors in P. aeruginosa. [Display omitted] •Design and synthesis of 31 novel amide derivatives as quorum sensing inhibitors (QSI) against P. aeruginosa.•The new compounds were screened for QSI activity, and compounds A9 and B6 exhibited anti-virulence activity.•Animal infection model experiment showed compound A9 can significantly improve their survival rate.•SPR and molecular docking studies demonstrated that A9 is a multi-targeted inhibitor that can bind to both LasR and PqsR.