Loss of GCN5L1 exacerbates damage in alcoholic liver disease through ferroptosis activation

Background and Aims Iron overload, oxidative stress and ferroptosis are associated with liver injury in alcohol‐associated liver disease (ALD), however, the crosstalk among these regulatory pathways in ALD development is unclear. Methods ALD mouse model and general control of amino acid synthesis 5 like 1 (GCN5L1) liver knockout mice were generated to investigate the role of GCN5L1 in ALD development. Proteomic screening tests were performed to identify the key factors mediating GCN5L1 loss‐induced ALD. Results Gene Expression Omnibus data set analysis indicates that GCN5L1 expression is negatively associated with ALD progression. GCN5L1 hepatic knockout mice develop severe liver injury and lipid accumulation when fed an alcohol diet. Screening tests identified that GCN5L1 targeted the mitochondrial iron transporter CISD1 to regulate mitochondrial iron homeostasis in ethanol‐induced ferroptosis. GCN5L1‐modulated CISD1 acetylation and activity were crucial for iron accumulation and ferroptosis in response to alcohol exposure. Conclusion Pharmaceutical modulation of CISD1 activity is critical for cellular iron homeostasis and ethanol‐induced ferroptosis. The GCN5L1/CISD1 axis is crucial for oxidative stress and ethanol‐induced ferroptosis in ALD and is a promising avenue for novel therapeutic strategies.