Cholesterol Interference in the Assessment of Vitamin D Status: A Canadian Health Measures Survey Biobank Project

Matrix effects are a known problem with immunoassays measuring serum 25-hydroxyvitamin D [25(OH)D]. To determine if the inverse association between serum 25(OH)D and serum cholesterol concentrations is a function of assay method: Diasorin Liaison 25(OH) Vitamin D Total Assay (Liaison Total Assay), an immunoassay, compared with liquid chromatography tandem mass spectrometry (LC-MS/MS). Canadian Health Measures Survey data and biobank serum (White males aged 20–79 y, n = 392) were evaluated for bias in serum 25(OH)D using Bland-Altman plots. Differences in serum 25(OH)D (Liaison Total Assay − LC-MS/MS) were compared among non-HDL-cholesterol