Construction and characterization of recombinant senecavirus A expressing secreted luciferase for antiviral screening

Senecavirus A (SVA) is a newly identified picornavirus associated with swine vesicular disease and neonatal mortality. The development of an SVA incorporating an exogenous reporter gene provides a powerful tool for viral research. In this study, we successfully constructed a recombinant SVA expressing Gaussia Luciferase (Gluc), termed rSVA-Gluc. The growth kinetics of rSVA-Gluc in BHK-21 cells were found to be comparable to those of the parental virus, and Gluc activity paralleled the virus growth curve. Genetic analysis revealed stable inheritance of the inserted reporter protein genes for at least six generations. We evaluated the utility of rSVA-Gluc in antiviral drug screening, and the results highlighted its potential as an effective tool for such purposes against SVA. The data that support the findings of this study are available on request from the corresponding author. •Generation and recovery of recombinant SVA expressing Gluc.•The recombinant virus exhibits proliferation characteristics on BHK-21 cells similar to the parental virus.•The recombinant virus rSVA-Gluc serves as an effective tool for antiviral drug screening.