CH4 control and nitrogen removal from constructed wetlands by plant combination

Global warming trend is accelerating. This study proposes a green and economical methane (CH4) control strategy by plant combination in constructed wetlands (CWs). In this study, a single planting of Acorus calamus L. hybrid constructed wetland (HCW-A) and a mixed planting of Acorus calamus L. and Eichhornia crassipes (Mart.) Solms hybrid constructed wetland (HCW-EA) were constructed. The differences in nitrogen removal performance and CH4 emissions between HCW-A and HCW-EA were compared and analyzed. The findings indicated that HCW-EA demonstrated significant improvements over HCW-A, with NH4+-N and TN removal rates increasing by 21.61% and 16.38% respectively, and CH4 emissions decreased by 43.36%. The microbiological analysis results showed that plant combination promoted the enrichment of Proteobacteria, Alphaproteobacteria and Bacillus. More nitrifying bacteria carrying nxrA genes and denitrifying bacteria carrying nirK genes accelerated the nitrogen transformation process. In addition, the absolute abundance ratio of pmoA/mcrA increased, reducing the release of CH4. [Display omitted] •Plant combination reduced CH4 emission by 43.36% with supporting higher pmoA/mcrA.•Plant combination increased the removal rates of NH4+-N and TN by 21.61% and 16.38%.•Plant combination increased the abundance of Proteobacteria, Alphaproteobacteria and Bacillus.•Plant combination enriched more nitrogen removal bacteria carrying nxrA and nirK gene.