Bosea beijingensis sp. nov., Telluria beijingensis sp. nov. and Agrococcus beijingensis sp. nov., isolated from baijiu mash

The baijiu fermentation environment hosts a variety of micro-organisms, some of which still remain uncultured and uncharacterized. In this study, the isolation, cultivation and characterization of three novel aerobic bacterial strains are described. The cells of strain REN20 were Gram-negative, strictly aerobic, motile and grew at 26-37 °C, at pH 6.0-9.0 and in the presence of 0-5.0 % (w/v) NaCl. The cells of strain REN29 were Gram-negative, strictly aerobic, motile and grew at 15-30 °C, at pH 6.0-9.0 and in the presence of 0-10.0 % (w/v) NaCl. The cells of strain REN33 were Gram-positive, strictly aerobic, motile and grew at 15-37 °C, at pH 5.0-10.0 and in the presence of 0-7.0 % (w/v) NaCl. The digital DNA-DNA hybridization and average nucleotide identity by orthology values between type strains in related genera and REN20 (20.3-36.8 % and 79.8-89.9 %), REN29 (20.3-36.8 % and 74.5-88.5 %) and REN33 (22.6-48.6 % and 75.8-84.2 %) were below the standard cut-off criteria for the delineation of bacterial species, respectively. Based on polyphasic taxonomy analysis, we propose three new species, sp. nov. (=REN20 =GDMCC 1.2894 =JCM 35118 ), sp. nov. (=REN29 =GDMCC 1.2896 =JCM 35119 ) and sp. nov. (=REN33 =GDMCC 1.2898 =JCM 35164 ), which were recovered during cultivation and isolation from baijiu mash.