Alginate Microbeads as Cell Support for Cartilage Tissue Engineering: Bioreactor Studies
Alginate was shown to be a suitable support for entrapment and cultivation of chondrocytes and bone marrow stromal cells, which under appropriate in vitro conditions synthesized cartilaginous components. The main limitation in these cultures may be low rates of mass transport through the alginate ma...
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Veröffentlicht in: | Materials science forum 2007-09, Vol.555, p.417-422 |
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Hauptverfasser: | , , , , |
Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Alginate was shown to be a suitable support for entrapment and cultivation of
chondrocytes and bone marrow stromal cells, which under appropriate in vitro conditions
synthesized cartilaginous components. The main limitation in these cultures may be low rates of
mass transport through the alginate matrix governed by diffusion. In this study, we have designed
and utilized a bioreactor system based on a packed bed of alginate beads with immobilized
chondrogenic cells. Continuous medium perfusion provided convective mass transport through the
packed bed, while small diameters of beads (2.5 mm and down to 500 μm) ensured short diffusion
distances to the immobilized cells. During up to 5 weeks of cultivation, the cells synthesized
extracellular matrix components merging beads together and indicating potentials of this system for
precise regulation of the cellular microenvironment in cartilage tissue engineering. |
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ISSN: | 0255-5476 1662-9752 1662-9752 |
DOI: | 10.4028/www.scientific.net/MSF.555.417 |