Automated high-throughput immunomagnetic separation-PCR for detection of Mycobacterium avium subsp. paratuberculosis in bovine milk

Two monoclonal antibody-mediated immunomagnetic separation PCR kits (AnDiaTec ParaTub-S® IMS-PCR-ELISA and ParaTub-SL® IMS-real time PCR) were developed and evaluated for the automated high-throughput detection of Mycobacterium avium subsp. paratuberculosis ( M. paratuberculosis) in bulk milk of nat...

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Veröffentlicht in:	International journal of food microbiology 2006-08, Vol.110 (3), p.201-208
Hauptverfasser:	Metzger-Boddien, Christoph, Khaschabi, Daryush, Schönbauer, Michael, Boddien, Sylvia, Schlederer, Thomas, Kehle, Johannes
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Automated automatic detection bacterial contamination Biological and medical sciences bulk milk Cattle Cattle Diseases - diagnosis Cattle Diseases - microbiology Colony Count, Microbial dairy herds enzyme-linked immunosorbent assay Female food contamination Food industries Food Microbiology food pathogens Fundamental and applied biological sciences. Psychology General aspects High-throughput immunomagnetic separation Immunomagnetic Separation - methods IMS-PCR M. paratuberculosis Methods of analysis, processing and quality control, regulation, standards microbial detection Milk - microbiology Mycobacterium avium Mycobacterium avium subsp. paratuberculosis Mycobacterium avium subsp. paratuberculosis - isolation & purification operating costs paratuberculosis Paratuberculosis - diagnosis Paratuberculosis - microbiology PCR-ELISA polymerase chain reaction Polymerase Chain Reaction - methods rapid methods Raw milk Real time PCR Sensitivity and Specificity
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